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Electron Microscopy Sciences

Specimen Preparation and Embedding Supplies

arrow14Molds, Chucks, and Holders

Butler Block Trimerarrow12Butler Block Trimer

A precision made device to aid in hand trimming resin tissue blocks to the desired shape for ultramicrotomy. The knurled knife guide swivels to help the trimming blade to cut the sides of the block. May be utilized with any stereomicroscope with epi-illumination. The trimmer is supplied with a standard chuck to accommodate the block size 00.  (Stain Technology, Vol. 49, No 4: pp. 129-132, 1974)

69945-01 BUTLER Block Trimming each 375.00 Add to Cart

Ultramicrotomy Chucksarrow12Ultramicrotomy Chucks

Precision machined from aluminum. These special chucks are made to accommodate small specimen blocks, which have been polymerized in Eppendorf tubes. In addition, we also have chucks that hold all sizes of flat embedding blocks (fitting all Leica and RMC instruments) ,and chucks for LKB instruments.

69990 Flat embedding Chuck each 435.00 Add to Cart
69991 LKB Chuck each 440.00 Add to Cart
69992 Eppendorf Chuck for RMC each 445.00 Add to Cart
69993 Eppendorf Chuck for LEICA each 445.00 Add to Cart

arrow12Chucks, Block Holders and Molding Cup Trays

arrow121. Aluminum Chucks

Machined from solid Aluminum. They come with set screws and a hex key.

Chucks, Block Holders and Molding Cup Trays

70174-10 ABH-JB Chucks for Hacker, A/O Reichert rotary and Autocut, JB-4 and JB-4A, LKB Ultratomes and Shandon Hypercut. Stem measures 11 x 11 mm each 66.00 Add to Cart
70174-14 ABH-LBK Huxley Chuck. Stem measures 11 x 25 mm each 96.00 Add to Cart
70174-20 ABH-JBA Chuck with a three point recessed block holder attachment: for LKB Histo Range and Reichert Super-Cut in addition to microtomes using ABH-JB chucks (#70174-10). Stem measures 11 x 11 mm  each 96.00 Add to Cart
Block Holderarrow122. EBH-2 Block Holder

Molded from hard plastic. EMS# 70175-50, 6m thick is shown to the right.

  • Easy to store block holders attached to Aluminum chucks (ABH)
  • Frosted surface for easy marking.
  • Large center hole prevents bubble formation.
70175-40 4mm Thin Embedding Stubs 100/pack 365.00 Add to Cart
70175-50 6mm Thick Embedding Stubs (shown above) 50/pack 132.00 Add to Cart
70175-60 10mm Thick Embedding Stubs, Mixed Colors 100/pack 365.00 Add to Cart
Molding Cup Tray arrow123. Molding Cup Tray

These polyethylene trays are ideal for casting specimen blocks embedded with JB-4 or equivalents and are used with our block holders and chucks shown above.

Catalog # Molding Cup Size Cavities Pk Price  
70176-10 6x12x5mm 20 each 67.00 Add to Cart
70176-15 12x16x5mm 20 each 67.00 Add to Cart
70176-20 6x8x5mm Hexagon 9 3/pk 85.00 Add to Cart
70176-25 2x15x5mm 9 3/pk 85.00 Add to Cart
70176-30 13x19x5mm 9 3/pk 85.00 Add to Cart

Polyethylene Moldsarrow12Polyethylene Molds - Individual

Molded from polyethylene, three popular sizes are available to meet your needs. These Individual Molds can be used with EBH2 Block Holders, #70175-50, 70175-60, or with colored stubs 70175-70.
Aluminum Embedding Tray is available with 6-position to hold these Individual Mold during polymerization. Comes with anodized blue and natural color.

Suitable for JB-4®, JB-4-Plus®, Immuno-Bed, LR White, LR Gold® or Paraffin embedding.

70177-12 Individual Mold, 12 x 8 mm 100/pack 121.00 Add to Cart
70177-16 Individual Mold, 16 x 8 mm 100/pack 121.00 Add to Cart
70177-19 Individual Mold, 19 x13 mm 100/pack 120.00 Add to Cart
70178-04 Embedding Tray, Aluminum, (2 Blue & 2 Natural colors) 4/pack 163.00 Add to Cart

Chien Silicone Universal-MoldChien Silicone Universal-Moldarrow12Chien Silicone Universal-Mold

A two-directional mold that allows for single embedded specimens to be sectioned both laterally and transversely . Tissues to be embedded are accurately trimmed and placed so that both sides of the tissue are against both edges of the cavity tip.

Chien, K., et al. (1985) Proc. Ann. EMSA Meeting 43, 460

70140 Chien Silicone Mold each 22.00 Add to Cart
70141 Chien Silicone Mold 10/lot 200.00 Add to Cart

Capsule Molds arrow12Capsule Molds

Our molds are made from the strongest possible silicone rubber providing extremely high tear resistance. They are all reusable and produce embedding blocks of numerous shapes and sizes with a wide variety of tips. We also offer a mold which produces blocks to be used for re-embedding. Choose the one that best suits your embedding needs!

70150 Capsule Molds Type A: 10 cavities, 8mm diameter body, 5mm diameter tip, 11mm height, tapered tip. each 13.00 Add to Cart
70160 Capsule Molds Type B: 12 cavities, 8mm diameterbody, 5mm diameter tip 16mm height, tapered tip. each 17.00 Add to Cart
70161 Capsule Molds Type C: 12 cavities cylindrical shapes, 8mm diameter 16mm height, flat tip. each 17.00 Add to Cart

Embedding Capsule SubstituteEmbedding Capsule Substitutearrow12The Embedding Capsule Substitute:

This 24 cavity mold produces a block similar to the embedding capsule block but with the following advantages: * Resin polymerizes better in silicone rubber than in polypropylene or polyethylene - the rubber heats more evenly and retains the heat better than in plastic.

  • It is easier to see inside the well when centering the specimen in the tip.
  • It is easier to remove the block from the well. There is no need for razor blades or press devices.
  • The finished blocks have finer tips so when you trim deeper into the block the face doesn't increase in size rapidly; therefore, constant retrimming is not necessary
70165 The Embedding Capsule Substitute each 22.00 Add to Cart

Mold for Long TissuesMold for Long Tissuesarrow12The Mold for Long Tissues:

This mold accommo dates longer pieces of tissues (muscle, skin biopsies, and many plant specimens)

Advantages:

  • Easy to embed long specimens and orient them properly. The tissues lie flat in the well; therefore, no horizontal trimming is needed.
  • It is ideal for any specimen that needs immediate sectioning (e.g. cell monolayer). 24 cavities
70166 The Mold for Long Tissues each 22.00 Add to Cart

Cell Culture MoldThe Cell Culture Mold: arrow12The Cell Culture Mold:

This mold is designed primarily for embedding cell culture specimens grown either directly on the bottom of a dish or onthe filter when cross sections of the cells are needed:

  1. Cells grown on petri dishes are cut into strips just before the propylene oxide dehydration step; when Propylene Oxide is added, the strips lift right off the bottom.
  2. When the strips are ready for embedment, they are rolled on an applicator stick into a wad and gently lowered into the well (the strips should not float).

Advantages:

  • Many cells can be examined in cross section.
  • There is no need for trimming - sectioning can begin immediately.
  • The curved side makes the tip very strong in spite of its thinness.
  • Easy detection of a strip of cells.
  • The label is placed on the round part so as not to interfere with the specimen.
  • 24 cavities.
70167 The Cell Culture Mold each 22.00 Add to Cart

Chang Monolayer Moldsarrow12Chang Monolayer Molds

Designed by Dr. J.P. Chang for the embedding of a monolayer of cells. This specially designed mold has proven useful in the processing of frozen sections, ascite cells, fresh imprints, as well as blood and marrow smears. J. of Ultrastructural Res., 37, 370 (1971).

70910 Chang Mold 12 cavities, 20x10x1mm each 10.00 Add to Cart
70920 Chang Mold 9 cavities, 20x20x1mm each 10.00 Add to Cart

Flat Embedding Moldsarrow12Flat Embedding Molds

A clear silicone rubber with very high tear strength. It can be reused more times than the conventional silicone molds. It is resistant to most epoxy resins and its clear base allows positioning of the specimen lighted from beneath.

arrow12Single Tapered Ends
70900 Standard flat embedding mold, 21 numbered cavities measures: 14mm(L)x5mm(W)x4mm(D). (Clear silicone) each 10.00 Add to Cart
70901 Same as #70900, but each of the 21 numbered cavities measures: 14mm(L)x5mm(W)x6mm(D). (White silicone) each 10.00 Add to Cart
70902 Same as #70900, but each of the 21 numbered cavities measures: 14mm(L)x5mm(W)x3mm(D). (White silicone) each 10.00 Add to Cart
arrow12Small Flat Embedding Mold

Made from clear silicone, the overall measurements are 1.65” x 3” with 12 cavities. Each cavity measures 0.427”(L) x 0.25”(W) x 0.156” Deep (10mmL x 6mmW x 4mm Deep). This mold produces 12 embedding blocks with tapered ends.

70903 Small Flat Embedding Mold each 9.00 Add to Cart
arrow12Double Tapered Ends

A standard flat embedding mold where both ends are tapered. This enables you to embed specimens in both ends of each casting. Made from white silicone, 21 numbered cavities, each cavity measuring: 14mm(L)x4mm(W)x4mm(D)

70870 Double Tapered Ends Mold each 10.00 Add to Cart
arrow12Triple Tapered Ends

A new design with 10 triple numbered, tapered tip cavities to save you time and handling. Holds firmly in a chuck during trimming or sectioning. Made from white silicone, each cavity measures 8mm from tip to center, 5mm deep.

70875 Triple Tapered Ends Mold each 16.50 Add to Cart

BEEM® Flat Embedding Moldsarrow12BEEM®*Flat Embedding Molds

Molded from polyethylene. The critical surfaces of the mold are transparent, facilitating accurate specimen orientation through bottom illumination. Molds are reusable and each mold is supplied with a protective box.

The cavity measures: 12mm(L) x 5mm(W) x 3mm(D)
Overall mold measures: 52mm(L) x 40mm(W) x 5mm(H)
Storage box measures: 21/3"(L)x1¾"(W)x½"(H) (57x45x12.5mm)

70904-01 BEEM® Flat Embedding Mold each 9.25 Add to Cart
70904-12 BEEM® Flat Embedding Mold 12/pk 96.00 Add to Cart

*BEEM® Is A Registered Trademark of Better Equipment For Electron Microscopy, Inc

Flat Embedding For Acrylicsarrow12Flat Embedding with Convenient Box

No more stickiness with new storage box

Most resins are no longer a challenge to flat embed when used in combination with the Cocoon box. The system withstands temperatures up to 70 Degrees C, and our flat embedding of many resins is made easier.

70900-CB 4mm Flat Embedding Mold w/Cocoon Box set 12.50 Add to Cart
70901-CB 6mm Flat Embedding Mold w/Cocoon Box set 12.50 Add to Cart
70902-CB 3mm Flat Embedding Mold w/Cocoon Box set 12.50 Add to Cart
70870-CB Double End Flat Embedding Mold w/ Cocoon Box set 12.50 Add to Cart

PTFE Embedding Moldtds PTFE Embedding Moldarrow12PTFE Embedding Mold

Machined from PTFE block. This mold offers flat embedding for  most resins, including LR White®, LR Gold®, JB-4, Immuno-Bed and Lowicryl®. This PTFE Flat Embedding Mold can be used for both UV/low temperature and thermal cure, and may be used as a substitution for the silicone molds. The individual cavities are pre shaped  to reduce block trimming.

For curing anaerobic material, we recommend using Aclar® film, cut-to-size of the mold and lower on top of the mold after you ‘just over-fill’ the cavities with embedding resin. Due to the flexibility of the PTFE, you just bent the mold to pop out the cured resin blocks. After all the cast blocks have been removed, reinsert the mold to its holder to re-shape the mold. The mold holder is machined from aluminum and anodized black.

Mold with 18 cavities, each cavity measures 5mm W x 14mmL x 4mm Deep

Overall mold measures: 5 ½” x 3” x ¼” (140x50x8mm)

Mold is available as a set (includes one PTFE mold and one aluminum holder).

70143 PTFE Flat Embedding Mold, set each $150.00 Add to Cart
70143-R PTFE Flat Embedding Mold only each $120.00 Add to Cart

Stepped Microtome Embedding Moldarrow12Stepped Microtome Embedding Mold

EMS Embedding Mold is a new mold designed with Michael J. Dykstra Ph.D. from North Carolina State University Department of Veterinary Medicine. This Mold produces blocks of the best dimension to be held securely by Reichert/Leica Ultracut stepped microtome chucks. There are 24 cavities and the depth of each cavity is 4mm which will prevent breakage of the blocks during razor blade trimming.

Mold Measurements: 3 3⁄8" x 3" (85 x 77mm)

Cavity Measurements: 15mmx 7mm x 4mm deep

Ordering

70907 Embedding Mold-Dykstra each 12.00 Add to Cart

plastic flat embedding moldarrow12Plastic Flat Embedding Mold

  • Injected molded polyethylene.
  • Resistant to most embedding materials.
  • Easy peeled-off the blocks.

Mold fits perfectly inside the cocoon box BE8 (64300-8); See Cocoon Box section.

When the mold and the box BE8 are used together, the set allows for the use of oxygen-prohibited embedding materials to be polymerized in a flat orientation. These molds are reusable. Each cavity measures 12mm(L) x 5mm(W) x 3.5mm Deep. Overall mold measures: 94 x 56 x 14mm.

70905-01 EMS Molded Flat Embedding Mold each 7.80 Add to Cart
70905-12 EMS Molded Flat Embedding Mold 12/pk 77.00 Add to Cart

Tissue Culture Monolayer Embedding arrow12Tissue Culture Monolayer Embedding Mold: Thompson Embedding Mold

This invention is a mold used for embedding mammalian cells in monolayer culture in preparation of sectioning for light or electron microscopy. Tissue culture monolayer embedding molds eliminate the need for traditional BEEM® capsules. The improved molds can be used to transfer the majority of cells from the entire surface of a cover slip to avoid losses of large amounts of cellular information occurring only randomly. In addition, other problems such as surface blemishes caused by air entrapment associated with BEEM® capsules are eliminated.

emsexclu.gif (210 bytes)Advantages:

  • The mold yields several individual blocks of resin from a single slip-cover.
  • The resin blocks fit directly into a chuck for flat specimens.
  • The embedded surfaces are free from blemishes caused by entrapped air.
  • The mold uses a minimum amount of resin.
70924 Thompson Mold each 12.00 Add to Cart
70925 Thompson Mold 4/lot 42.00 Add to Cart

Slide Duplicating Moldsarrow12emsexclu.gif (210 bytes)Slide Duplicating Molds

Uses a 1x3" glass slide as a base, and duplicates the slide using the resin of your choice. Tissue sections are embedded against the flat glass surface. This duplicated resin slide can be studied under the Light Microscope, then cut, remounted, resectioned, and studied under the EM. A three cavity mold, each cavity measuring: 1"(W)x3"(L)x3/32"(D). Reference & Instructions enclosed

70170 Slide Duplicating Mold each 15.00 Add to Cart

Technical Tip

A Method for Slide Embedding

Slide Duplicating Moldsarrow12Slide Duplicating With Cover Slip Mold

The same design as above 70170 but with the addition of three round cavities which are 17mm in diameter and 1mm deep.

70172 Slide Duplicating w/Cover Slip Mold each 17.00 Add to Cart

Weighing Boats, Canoes, Dishes, and Paper arrow12arrow12