Eyepiece Graticules

The standard sizes of eyepiece graticules used to be 16, 19, and 21 mm. For your convenience, this has been greatly expanded to accommodate a variety of equipment without the need to special order. The following table will assist you in determining appropriate eyepieces for common brands.

Brand	Reticle Diameter	Marking on Eyepiece
Olympus Eyepieces	24 mm	WHN10X, WHN10X-H	WH10X, WH10X-H, WH15X
		WHS10X-H, WHS15X-H	CWH10X, CWH10X-H, CWH30X
		GW10X	GWH10X
		GSWH10X, GSWH15X, GSWH20X, GSWH30X	WH12.5X, WH12.5X-H
		WHSZ10X-H, WHSZ15X-H, WHSZ20X-H, WHSZ30X-H	GSWH10X-H, GSWH15X-H, GSWH20X-H, GSWH30X-H
	20.4 mm	WHK8X, WHK10X, WHK15X	PE 2.5X/ 3.3X/ 4X/ 5X
		NWHK10X, (*WHK10X-H cannot be installed)	GWH15X-D, GWH15X-CD
		NFK 2.5X/ 3.3X/ 5X/ 6.7X
Nikon Eyepieces	27 mm	CFI 10x, CFI 12.5x, CFI 15x, CFI UW 10x	CFUW10X, CFUWN 10X
		E1-CFI 10x, E2-CFI 10x, E2-CFI 15x	SME 10x /23, SME 10x /21
		SMZ 10 UW15x /17, SMZ 10 UW20x/15
	25 mm	C-W 10x B/22 (C-W 10X A/22)	L-W10x ESD (FOV 22)
		SMZ U UW10x A/24, SMZ U UW15x
Leica Eyepieces	26 mm	11507807 (S10x/22B.M)	11507808 (S10x/25Br.M)
		11507801 (10x/20Br.M)	11507802 (10x/20Br.M)
		11506515 (12.5x/16Br.M)
	23 mm	10447160 (10x/21B)	10445301 (16x/14B)
		10445302 (25x/9.5B)	10445303 (40x/6B)
	24.5 mm	10450023 (10x/23B)	10450024 (16x /15B)
		10450025 (25x/9.5B)	10450026 (40x/6B)
		10 446 333 (10x/23, adjustable)	13 613 532 (10x/20, focusing)
		10 446 355 (16x/16, adjustable)	10 446 357 (20x/12, adjustable)
		10 446 329 (10x/23B, adjust.f/eyeglasses)	10 447 131 (10x/23, adjustable)
		10 447 133 (16x/16, adjustable)	10 447 135 (20x/126, adjustable)
		10 447 137 (for eyeglasses10x/23B, adjustable)	10 447 139 (for eyeglasses16x/15B, adjustable)
		10 445 302 (25x/9.5B, adjust.f/eyeglasses)	10 445 303 (40x/6B, adjust.f/eyeglasses)
		10 447 280 (10x/20B, adjust.f/eyeglasses)
Zeiss Eyepieces	26 mm	E-PL 10x/20 Br. Foc	PL 10x/21 Br Foc
		PL 10x/23 Br. Foc
	23 mm	WF 10x/18	WF 10X/20
	21 mm	PL16x/16 Br. Foc	W 25x/10 foc
		Kpl-W 10x/18 [#46 40 43-9902]
This list is not comprehensive so please inquire if your eyepiece or brand is not listed.

A. Size and Position Scales

Single Lines - NE50

For measurement of large objects in conjunction with a graduated mechanical stage, and for alignment as well. Image covers entire field of view.

Pattern	Description	Dia	Cat #	Qty	Price
NE50	Single Line, nominal width 0.02 mm. Surface chrome image.	16 mm	67982-16	each	101.00	Add to Cart
		19 mm	67982-19	each	101.00	Add to Cart
		21 mm	67982-21	each	101.00	Add to Cart

Cross Lines - NE8, NE81, NE82

Used as NE50 but for measurements in two directions and for sighting and alignment. Image covers the entire field of view.

Pattern	Description	Dia	Cat #	Qty	Price
NE8	Cross lines with surface chrome image. Nominal line width 0.02 mm.	16 mm	67983-01	each	101.00	Add to Cart
		19 mm	67983-05	each	101.00	Add to Cart
		20.4 mm	67983-20.4	each	101.00	Add to Cart
		21 mm	67983-10	each	101.00	Add to Cart
		23 mm	67983-23	each	101.00	Add to Cart
		24.5 mm	67983-24.5	each	101.00	Add to Cart
		25 mm	67983-025	each	101.00	Add to Cart
		26 mm	67983-26	each	101.00	Add to Cart
		27 mm	67983-27	each	101.00	Add to Cart
NE81	Cross lines with surface chrome image. Nominal line width 0.04 mm.	16 mm	67983-20	each	101.00	Add to Cart
		19 mm	67983-25	each	101.00	Add to Cart
		21 mm	67983-30	each	101.00	Add to Cart
NE82	Cross lines with surface chrome image. Nominal line width 0.005 mm.	16 mm	67984-16	each	101.00	Add to Cart
		19 mm	67984-19	each	101.00	Add to Cart
		21 mm	67984-21	each	101.00	Add to Cart

Broken Cross Lines - NE56

Used as above. Broken lines enable fine detail to be seen at the breaks. A thin boundary would be lost behind a a continuous line. Image covers the entire field of view.

Pattern	Description	Dia	Cat #	Qty	Price
NE56	Broken cross lines. Surface chrome image.	16 mm	67985-16	each	101.00	Add to Cart
		19 mm	67985-19	each	101.00	Add to Cart
		21 mm	67985-21	each	101.00	Add to Cart

Crossed Gauge Lines - NE53, N54

Use as crossed lines, but for the measuring of distances between the lines. Greater accuracy can be obtained by locating the specimen detail between the graticules gauge pair.

Pattern	Description	Dia	Cat #	Qty	Price
NE53	Two vertical lines 0.10 mm apart with horizontal line. Image covers the entire field of view. Surface chrome image.	16 mm	67986-16	each	121.00	Add to Cart
		19 mm	67986-19	each	121.00	Add to Cart
		21 mm	67986-21	each	121.00	Add to Cart
NE54	Two vertical lines 0.2 mm apart.	16 mm	67975-16	each	110.00	Add to Cart
		19 mm	67975-19	each	110.00	Add to Cart
		21 mm	67975-21	each	110.00	Add to Cart
		Custom	67975-50	each	205.00	Add to Cart

Horizontal & Vertical Scales - NE1, NE2, NE5, NE20, NE28, NE31, NE41, NE120

These scales are used for the measuring of lengths of specimen or distances between points on a variety of different shaped objects. NE1 scale shown: This eyepiece graticule has an overall length of 10.00 mm with 100 subdivisions of 0.1 mm. When used with a x10 objective, each division will represent 10 microns on the specimen. By dividing the division of the chosen graticule by the magnification of the objective, one obtains an approximate value that each division will represent on the stage.

Pattern	Description	Dia	Cat #	Qty	Price
NE1	Horizontal micrometer scale 10 mm long with 0.1 mm subdivisions. Surface chrome image.	16 mm	68010-16	each	98.00	Add to Cart
		19 mm	68010-19	each	98.00	Add to Cart
		20.4 mm	68010-20.4	each	98.00	Add to Cart
		21 mm	68010-21	each	98.00	Add to Cart
		23 mm	68010-23	each	98.00	Add to Cart
		24.5 mm	68010-24.5	each	124.00	Add to Cart
		25 mm	68010-25	each	124.00	Add to Cart
		26 mm	68010-26	each	124.00	Add to Cart
		27 mm	68010-27	each	124.00	Add to Cart
NE2	Vertical micrometer scale 10 mm long with 0.1 mm subdivisions. Surface chrome image.	16 mm	68006-16	each	112.00	Add to Cart
		19 mm	68006-19	each	112.00	Add to Cart
		21 mm	68006-21	each	112.00	Add to Cart
NE5	Horizontal micrometer scale 5 mm long with 0.05 mm subdivisions. Surface chrome image.	16 mm	67988-16	each	112.00	Add to Cart
		19 mm	67988-19	each	112.00	Add to Cart
		21 mm	67988-21	each	112.00	Add to Cart
NE20	Horizontal scale 0.10 inch long with 100 subdivisions. Surface scale image.	16 mm	67987-16	each	112.00	Add to Cart
		19 mm	67987-19	each	112.00	Add to Cart
		21 mm	67987-21	each	112.00	Add to Cart
NE28	Horizontal micrometer scale. 1 mm long with 100 subdivisions. Surface chrome image.	16 mm	67992-16	each	121.00	Add to Cart
		19 mm	67992-19	each	121.00	Add to Cart
		21 mm	67992-21	each	121.00	Add to Cart
NE31	Horizontal scale 0.5" long with 100 divisions of 0.0005"	16 mm	67977-16	each	105.00	Add to Cart
		19 mm	67977-19	each	105.00	Add to Cart
		21 mm	67977-21	each	105.00	Add to Cart
		Custom	67977-50	each	207.00	Add to Cart
NE41	Horizontal micrometer scale 10 mm long with 200 subdivisions. Surface chrome image.	16 mm	67993-16	each	121.00	Add to Cart
		19 mm	67993-19	each	121.00	Add to Cart
		21 mm	67993-21	each	121.00	Add to Cart
NE120	Horizontal Micrometer 20 mm long with 100 divisions of 0.10 mm.	21 mm	67976-21	each	105.00	Add to Cart
		Custom	67976-50	each	210.00	Add to Cart

Crossed Scales - NE17, NE18, NE70, NE72

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern	Description	Dia	Cat #	Qty	Price
NE17	Crossed micrometer scales 10 mm long with 0.1 mm subdivisions. Surface chrome image.	16 mm	67989-16	each	122.00	Add to Cart
		19 mm	67989-19	each	122.00	Add to Cart
		20.4 mm	67989-20.4	each	122.00	Add to Cart
		21 mm	67989-21	each	122.00	Add to Cart
		23 mm	67989-23	each	122.00	Add to Cart
		24.5 mm	67989-24.5	each	126.00	Add to Cart
		25 mm	67989-25	each	126.00	Add to Cart
		26 mm	67989-26	each	126.00	Add to Cart
		27 mm	67989-27	each	126.00	Add to Cart
NE18	Crossed micrometer scales 5 mm long with 0.05 mm subdivisions. Surface chrome image.	16 mm	67994-16	each	121.00	Add to Cart
		19 mm	67994-19	each	121.00	Add to Cart
		21 mm	67994-21	each	121.00	Add to Cart
NE72	Crossed Micrometer scale 20 mm long with 200 divisions of 0.10 mm.	23 mm	67978-23	each	105.00	Add to Cart
		24 mm	67978-24	each	207.00	Add to Cart
		26 mm	67978-26	each	149.00	Add to Cart
		27 mm	67978-27	each	149.00	Add to Cart
		Custom	67978-50	each	187.00	Add to Cart
NE70	Crossed Micrometer scale (Imperial). 0.8' long with 400 divisions of 0.002".	21 mm	67978-21	each	105.00	Add to Cart
		23 mm	67979-23	each	186.00	Add to Cart
		24 mm	67979-24	each	149.00	Add to Cart
		26 mm	67979-26	each	149.00	Add to Cart
		27 mm	67979-27	each	149.00	Add to Cart
		Custom	67979-50	each	186.00	Add to Cart

Scales with Cross Lines - NE7, NE77, NE777

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern	Description	Dia	Cat #	Qty	Price
NE7	Horizontal micrometer scale 10 mm long with cross lines at 0.10 mm spacing. Surface chrome image.	16 mm	68013-16	each	112.00	Add to Cart
		19 mm	68013-19	each	112.00	Add to Cart
		20.4 mm	68013-20.4	each	112.00	Add to Cart
		21 mm	68013-21	each	112.00	Add to Cart
		23 mm	68013-23	each	112.00	Add to Cart
		24.5 mm	68013-24.5	each	140.00	Add to Cart
		25 mm	68013-25	each	140.00	Add to Cart
		26 mm	68013-26	each	140.00	Add to Cart
		27 mm	68013-27	each	140.00	Add to Cart
NE7N	Horizontal scale 10 mm/0.10 mm + crossline + square. Surface chrome image.	16 mm	67979-16	each	121.00	Add to Cart
		19 mm	67979-19	each	121.00	Add to Cart
		21 mm	67979-21	each	121.00	Add to Cart
NE77	Horizontal micrometer scale 5 mm long in 100 divisions with cross line. Surface chrome image.	16 mm	68012-16	each	112.00	Add to Cart
		19 mm	68012-19	each	112.00	Add to Cart
		21 mm	68012-21	each	112.00	Add to Cart
NE777	Horizontal micrometer scale 0.5" long with divisions of 0.005" and cross lines.	16 mm	67980-16	each	107.00	Add to Cart
		19 mm	67980-19	each	107.00	Add to Cart
		21 mm	67980-21	each	107.00	Add to Cart
		Custom	67980-50	each	218.00	Add to Cart

B. Squares and Grids

Note: These scales may need to be calibrated, according to intended use. There are a number of uses for the grids and squares listed and they will largely depend on the individual user's application.

Eyepiece Scales

• Sectoring: A squared graticule might be used for the systematic examination of a specimen. Some of the squared patterns are numbered to aid in the identification of areas of interest. Sectioning is particularly useful for making drawings of specimens onto graph paper. The chess-board type of pattern helps the user to distinguish the position being examined: the darker squares are translucent, while the lighter ones are transparent, avoiding eyestrain in prolonged counting as may be necessary in hematology. These patterns provide the same advantages when used with image analysis and capture devices.
• Counting: A squared graticule can be used for counting. Here, the basic principle is that a small area of the specimen is analyzed in order to obtain information about the total area. This minimizes wasteful work, enabling simple analysis of a paricular area. An example of this would be the comparison of large to small particles in a specimen. By using the Miller graticule (NE57) only the smaller particles in the small square are counted, the result being multiplied by ten for comparison with the number of larger particles in the large square.
• Squared Grids: Squared grids can be used in particle size analysis as simple technical aids where sophisticated image analysis systems are not required. The areas of the particles to be measured can be estimated by simply counting the number of squares occupied by those particles. It is necessary to estimate fractions of a square or make a rule (e.g. count as a square all partly covered squares at the right and bottom sides of the grid, and ignore partly covered squares at the left and upper sides of the squares). This method would only be useful for a fairly crude estimation of a large diameter.

Squared Grids - NE10, NE11, NE34

Simple grids are convenient for making sketches of the obseved speimen on graph paper. They are also useful for particle counting.

Pattern	Description	Dia	Cat #	Qty	Price
NE10	Grid (net) 0.50 mm pitch (distance of center bar to center bar). Surface chrome image.	16 mm	68011-16	each	112.00	Add to Cart
		19 mm	68011-19	each	112.00	Add to Cart
		21 mm	68011-21	each	112.00	Add to Cart
NE11	Grid (net) 1.00 mm pitch (distance of center bar to center bar). Surface chrome image.	16 mm	68015-16	each	118.00	Add to Cart
		19 mm	68015-19	each	118.00	Add to Cart
		21 mm	68015-21	each	118.00	Add to Cart
NE34	Grid (net) 0.10 mm squares. Surface chrome image.	16 mm	68018-16	each	124.00	Add to Cart
		19 mm	68018-19	each	124.00	Add to Cart
		21 mm	68018-21	each	124.00	Add to Cart

Indexed Grids - NE10A, NE11A, NE34A, NE71

Useful for particle counting, particularly when reference is needed between workers. Also, they are useful for area of specimen determinations.

Pattern	Description	Dia	Cat #	Qty	Price
NE10A	Numbered grid 5 mm x 5 mm, 0.5 mm pitch. Marked 1-10 and A-J. Surface chrome image.	16 mm	68011-16A	each	112.00	Add to Cart
		19 mm	68011-19A	each	112.00	Add to Cart
		21 mm	68011-21A	each	112.00	Add to Cart
NE11A	Numbered grid 10 mm x 10 mm, 1.0 mm pitch. Marked 1-10 and A-J. Surface chrome image.	16 mm	68016-16	each	95.00	Add to Cart
		19 mm	68016-19	each	95.00	Add to Cart
		20.4 mm	68016-20.4	each	95.00	Add to Cart
		21 mm	68016-21	each	95.00	Add to Cart
		23 mm	68016-23	each	95.00	Add to Cart
		24.5 mm	68016-24.5	each	116.00	Add to Cart
		25 mm	68016-25	each	116.00	Add to Cart
		26 mm	68016-26	each	116.00	Add to Cart
		27 mm	68016-27	each	116.00	Add to Cart
NE34A	Numbered grid 1 mm x 1 mm, 0.1 mm pitch. Marked 1-10 and A-J. Surface chrome image.	16 mm	68018-16A	each	124.00	Add to Cart
		19 mm	68018-19A	each	124.00	Add to Cart
		21 mm	68018-21A	each	124.00	Add to Cart
NE71	Index Pattern 20x20. Grid of 0.50 mm squares.	21 mm	67981-21	each	107.00	Add to Cart
		23 mm	67981-23	each	107.00	Add to Cart
		24 mm	67981-24	each	150.00	Add to Cart
		26 mm	67981-26	each	150.00	Add to Cart
		Custom	67981-50	each	192.00	Add to Cart

Indexed Grids - NE35

Useful for area of specimen determinations, especially rectangular shapes, also for particle counting.

Pattern	Description	Dia	Cat #	Qty	Price
NE35	Numbered grid 10 mm x 10 mm. 1 mm indexed squares. Surface chrome image.	16 mm	68014-16	each	118.00	Add to Cart
		19 mm	68014-19	each	118.00	Add to Cart
		21 mm	68014-21	each	118.00	Add to Cart

Chessboard Squares - NE15

The dark squares are translucent. Used as an alternative to simple grids for area of specimen determination and particle counting. Alternative light and dark squares help reduce eyestrain.

Pattern	Description	Dia	Cat #	Qty	Price
NE15	Chessboard (net) 2.0 mm squares. Surface chrome image.	16 mm	68008-16	each	236.00	Add to Cart
		19 mm	68008-19	each	236.00	Add to Cart
		21 mm	68008-21	each	236.00	Add to Cart

Squares - NE38

Combines three areas in one for convenience, giving area ratios A:B of 1:3 and B:C of 1:2.

Pattern	Description	Dia	Cat #	Qty	Price
NE38	Squares 10 mm, 7 mm & 4 mm. Glass sandwich.	16 mm	68017-16	each	124.00	Add to Cart
		19 mm	68017-19	each	124.00	Add to Cart
		21 mm	68017-21	each	124.00	Add to Cart

Miller Squares - NE57

The ratio of large to small squares is 9:1. Originally designed for hematology, they can be utilized for rapid counting of any evenly spread field of particle.

References

• American J. of Clin. Pathol. Vol. 20, 1950, page 1079. "Time Saving Device For Counting Reticulocyte." G.Brescher and Schneiderman.
• Practicle Haemotology. J.D. Dacy. Published by J.A. Churchill. 2nd Edition 1956 page 25.

Pattern	Description	Dia	Cat #	Qty	Price
NE57	Miller 7 x 7 mm grid. Glass sandwich.	16 mm	68020-16	each	124.00	Add to Cart
		19 mm	68020-19	each	124.00	Add to Cart
		20.4 mm	68020-20.4	each	124.00	Add to Cart
		21 mm	68020-21	each	124.00	Add to Cart
		23 mm	68020-23	each	124.00	Add to Cart
		24.5 mm	68020-24.5	each	135.00	Add to Cart
		25 mm	68020-25	each	135.00	Add to Cart
		26 mm	68020-26	each	135.00	Add to Cart
		27 mm	68020-27	each	135.00	Add to Cart

Whipple Grid - NE29

Originally designed for water particle analysis, but may be used for other aspects of particle counting. Grid shown: Ratio of full square to smallest is 50:1. Area is 2500:1.

Reference

• Microscopy of Drinking Water.

Pattern	Description	Dia	Cat #	Qty	Price
NE29	Whipple grid 100 squares in 7 mm area. Surface chrome image.	16 mm	68009-16	each	126.00	Add to Cart
		19 mm	68009-19	each	126.00	Add to Cart
		20.4 mm	68009-20.4	each	126.00	Add to Cart
		21 mm	68009-21	each	126.00	Add to Cart
		23 mm	68009-23	each	126.00	Add to Cart
		24.5 mm	68009-24.5	each	126.00	Add to Cart
		25 mm	68009-25	each	140.00	Add to Cart
		26 mm	68009-26	each	140.00	Add to Cart
		27 mm	68009-27	each	140.00	Add to Cart

C. Gauges

Concentric Circles - NE42, NE43, NE44, NE47

Can be used for two-way measurement when calibrated as a micrometer.

Pattern	Description	Dia	Cat #	Qty	Price
NE42	Concentric circle 0.25 mm - 2.5 mm dia. 10 circles. Surface chrome image.	16 mm	68019-16	each	124.00	Add to Cart
		19 mm	68019-19	each	124.00	Add to Cart
		21 mm	68019-21	each	124.00	Add to Cart
NE43	Concentric circle 0.5 mm - 5 mm dia. 10 circles. Surface chrome image.	16 mm	67997-16	each	121.00	Add to Cart
		19 mm	67997-19	each	121.00	Add to Cart
		21 mm	67997-21	each	121.00	Add to Cart
NE44	Concentric circle 1 mm - 10 mm dia. 10 circles. Surface chrome image.	16 mm	67998-16	each	121.00	Add to Cart
		19 mm	67998-19	each	121.00	Add to Cart
		20.4 mm	67998-20.4	each	121.00	Add to Cart
		21 mm	67998-21	each	121.00	Add to Cart
		23 mm	67998-23	each	121.00	Add to Cart
		24.5 mm	67998-24.5	each	121.00	Add to Cart
		25 mm	67998-25	each	121.00	Add to Cart
		26 mm	67998-26	each	121.00	Add to Cart
		27 mm	67998-27	each	121.00	Add to Cart
NE47	Concentric circle 2 mm - 20 mm dia. 10 circles. Surface chrome image.	16 mm	67999-16	each	121.00	Add to Cart
		19 mm	67999-19	each	121.00	Add to Cart
		21 mm	67999-21	each	121.00	Add to Cart

Concentric Circles and Cross Scales - NE48

Pattern	Description	Dia	Cat #	Qty	Price
NE48	Concentric circles. 10 circles, 1 mm-10 mm with graduated cross hairs.	16 mm	67991-16	each	118.00	Add to Cart
		19 mm	67991-19	each	118.00	Add to Cart
		21 mm	67991-21	each	118.00	Add to Cart
		23 mm	67991-23	each	118.00	Add to Cart
		Custom	67991-50	each	238.00	Add to Cart

Concentric Circle - NE22

This design leaves the circle clear of obstruction. In addition the intermediate lines are broken to improve ease of reading.

Pattern	Description	Dia	Cat #	Qty	Price
NE22	Concentric circle 0.5 mm - 12 mm dia. 24 circles. Surface chrome image.	16 mm	68000-16	each	121.00	Add to Cart
		19 mm	68000-19	each	121.00	Add to Cart
		21 mm	68000-21	each	121.00	Add to Cart

Gauge Pairs - NE19

Gauge pairs occupying a field of view of 10 mm. Each gauge is proportional to its adjacent number. The approximately size of the smallest pair = 0.1 mm.

Pattern	Description	Dia	Cat #	Qty	Price
NE19	Gauge pairs. Surface chrome image.	16 mm	68001-16	each	121.00	Add to Cart
		19 mm	68001-19	each	121.00	Add to Cart
		21 mm	68001-21	each	121.00	Add to Cart

Protractors - NE25, NE45

Pattern	Description	Dia	Cat #	Qty	Price
NE25	Half protractor scale. 10 mm diameter divided in degrees. Surface chrome image.	16 mm	67996-16	each	126.00	Add to Cart
		19 mm	67996-19	each	126.00	Add to Cart
		21 mm	67996-21	each	126.00	Add to Cart
NE45	Full protractor scale. 10 mm diameter divided in degrees. Surface chrome image.	16 mm	68002-16	each	121.00	Add to Cart
		19 mm	68002-19	each	121.00	Add to Cart
		21 mm	68002-21	each	121.00	Add to Cart

D. Particle Sizing and Distributing

The use of the eyepiece graticules shown in this section makes it possible to analyze specimens containing particles as an alternative, or in addition to, sieving. Graticules for particle size analysis are particularly popular when there are only limited quantities of particles or where particles are smaller than 50 microns in diameter. Typical substances analyzed are sand grains, soil particles, plant seeds, fertilizers, abrasives, liquid droplets, pigments, pulverized coal, silica, fibers, and fine dust.

The basic principle employed is to compare particles to the globes and circles of varying sizes that appear on the graticule – dark particles being compared to solids globes, and light or transparent ones to the circles. Naturally the procedure varies with the graticule concerned, more information about which is given below.

Please note that for calibration, the circles and globes will represent particles smaller in diameter by the magnification of the objective.

Paterson Globes and Circles - NG1

The graticule consists of a central rectangle, sub-divided into nine smaller rectangles, with a number of increasing circles outside the top and bottom horizontal edges. The marked figures are the diameters of the circles in units. 250 units represent the horizontal length of the large rectangle. Rectangle size is 4.5 mm x 2.025 mm. Circle sizes in microns are 450, 360, 270, 225, 180, 145, 110, 74, 37, and 18.

Pattern	Description	Dia	Cat #	Qty	Price
NG1	Paterson globes/circles. Surface chrome image.	16 mm	68031-16	each	225.00	Add to Cart
		19 mm	68031-19	each	225.00	Add to Cart
		21 mm	68031-21	each	225.00	Add to Cart

Porton - NG2

The circle areas of the Porton greaticules increases with Root 2 progression, as do the divisions on the right hand side of the rectangle. These divisions are numbered for convenience. The rectangle size is 4.5 mm x .25 mm. Circle sizes in microns are 560, 400, 280, 200, 140, 100, 70, 50, and 35. The specimen is racked on the mechanical stage of the microscope and traverses are taken right across the deposit, sizing all the particles encountered.

Pattern	Description	Dia	Cat #	Qty	Price
NG2	Original Porton globes/circles. Surface chrome image.	16 mm	68024-16	each	248.00	Add to Cart
		19 mm	68024-19	each	232.00	Add to Cart
		21 mm	68024-21	each	232.00	Add to Cart

New Porton - NG12

The NG12 is particularly useful since the array of globes and circles are conveniently close to where the particles pass. At the end of each band of the sample the mechanical stage is traversed vertically to take in the next band until the whole sample has been covered.

Pattern	Description	Dia	Cat #	Qty	Price
NG12	Modified Porton pattern globes/circles. Surface chrome image.	16 mm	68032-16	each	225.00	Add to Cart
		19 mm	68032-19	each	225.00	Add to Cart
		21 mm	68032-21	each	225.00	Add to Cart

British Standard Graticule - NG10

In this graticule the circle areas double progressively, hence the diameters alter by Root 2, so that the size classes can form a continuation of the standard series of sieves for particle sizing. Each particle is assigned to a size class defined by two adjacent circles, which represent the size limits of that class. Thus the distribution of size is obtained in terms of the diameter of circles having the same projected area as the particles. This method will cover particles in the range 150 micron to 0.38 micron. The size distributions with respect to their number and weight are determined separately. Final results are calculated as cumulative percents.

Originally designed by the National Coal Board for use in coal mining.

Pattern	Description	Dia	Cat #	Qty	Price
NG10	British Standard (BS3625/BS3260) Globes & Circles. Surface chrome image.	16 mm	68026-16	each	248.00	Add to Cart
		19 mm	68026-19	each	248.00	Add to Cart
		21 mm	68026-21	each	248.00	Add to Cart

Fairs - NG5

Designed to extend the sizing range of globe and circle graticules.

Example: Used in conjunction with NG2 the overall size range = 128:1. The circles increase by 2.

Note: Both graticules would have to be used with the same microscope, eyepiece and objective.

Pattern	Description	Dia	Cat #	Qty	Price
NG5	Fairs, grid length 4 mm. Glass Sandwich.	16 mm	68033-16	each	225.00	Add to Cart
		19 mm	68033-19	each	225.00	Add to Cart
		21 mm	68033-21	each	225.00	Add to Cart

E. Asbestos Fiber Analysis

Walton and Beckett Graticule for Asbestos - G22, G24, G25

Calibration factors are required for each of these graticules. See note below.

The Walton and Beckett graticule is used for counting fibrous dust (e.g. asbestos or glass fibers) and is particularly useful where the majority of fibers to be counted are shorter than 5 microns. The circle is divided into four by two diametrical lines scaled in units of 5 and 3 microns respectively. 3 and 5 microns are the critical measurements of fiber lengths and diameters used in fiber counting. Unlike the usual globes of other particle graticules, the Walton and Beckett have a series of shapes to compare objects with. These shapes have been designed for comparison with fibers, especially since they incorporate an aspect ratio of 3:1 or 5:1, essential for such analysis.

Important Note: The circle on these Walton & Beckett graticules must represent 100 microns at the stage and each one must be manufactured to suit the individual instrument.

Therefore, the following details should be provided with your order:

1. Calibration factor, if known
2. Objective magnification
3. Eyepiece magnification
4. Diameter of graticule disc required
5. Microscope make and model

Pattern	Description	Dia	Cat #	Qty	Price
G22	Walton & Beckett for asbestos, 3:1 ratio. Glass sandwich.	16 mm	68028-16	each	280.00	Add to Cart
		19 mm	68028-19	each	280.00	Add to Cart
		20.4 mm	68028-20.4	each	280.00	Add to Cart
		21 mm	68028-21	each	280.00	Add to Cart
		23 mm	68028-23	each	280.00	Add to Cart
		24.5 mm	68028-24.5	each	280.00	Add to Cart
		25 mm	68028-25	each	280.00	Add to Cart
		26 mm	68028-26	each	280.00	Add to Cart
		27 mm	68028-27	each	280.00	Add to Cart
G24	Walton & Beckett for asbestos, 5:1 ratio. Glass sandwich.	16 mm	68029-16	each	280.00	Add to Cart
		19 mm	68029-19	each	280.00	Add to Cart
		21 mm	68029-21	each	280.00	Add to Cart
G25	Based on the G22, the G25 is produced by the Institute of Occupational Health (1996). Glass sandwich.	16 mm	68030-16	each	280.00	Add to Cart
		19 mm	68030-19	each	280.00	Add to Cart
		21 mm	68030-21	each	280.00	Add to Cart

F. Specialist Designs

Spray Droplet Sizing Reticle (Matthews) - NG30

For size and distribution assessments of aerosol droplets. Used in conjunction with a 40x Microscope for direct measurements of droplets from 50-400 micron diameters. The actual pattern sizes are 50, 100, 200, and 400 microns. W.H.O. (Details on Request) and G.A. Matthews. Imperial College.

Pattern	Description	Dia	Cat #	Qty	Price
NG30	Matthews Spray Droplet	16 mm	68003-16	each	260.00	Add to Cart
		19 mm	68003-19	each	260.00	Add to Cart
		21 mm	68003-21	each	260.00	Add to Cart
		23 mm	68003-23	each	260.00	Add to Cart
		Custom	68003-50	each	330.00	Add to Cart

Thompson - G23

For counting particles in any of three areas of known size. The graticule is calibrated in the same manner as a normal eyepiece scale. The result is then used to calculate the area of any square.

Pattern	Description	Dia	Cat #	Qty	Price
G23	Thompson for dust analysis. 10 mm, 7 mm and 4 mm squares. Glass Sandwich.	16 mm	68090-16	each	282.00	Add to Cart
		19 mm	68090-19	each	282.00	Add to Cart
		21 mm	68090-21	each	282.00	Add to Cart

Chalkley Point Array - NG52

This is used to quickly determine the relationship of components to each other using random sampling. Curtis gives an example of its application, where a researcher may want to see whether or not a certain drug affects the volume proportion of cell types in a given organ. With this graticule the proportion of points lying over the image of one type of component is statistically proportional to the area occupied by that component. The 25 points of the array are placed over the field of view at random, so that a comparison can be made between the number of points touching one type of component with the number touching the other type of component in each viewing. A series of observations will yield an increasingly accurate ratio of the comparative incidence of each type of particle.

Reference

• A.S.C. Curtis. Medical and Biological Illustration, Vol. 10. pp 261-266. "Area and Volume Measurements by Random Sampling Methods."

Pattern	Description	Dia	Cat #	Qty	Price
NG52	Chalkley point array. Surface chrome image.	16 mm	68034-16	each	244.00	Add to Cart
		19 mm	68034-19	each	244.00	Add to Cart
		21 mm	68034-21	each	244.00	Add to Cart

Pharmaceutical PSA Pattern - G57

This graticule was designed for the pharmaceutical industry. However, it is also useful where particle size considerations are restricted to 10 mm and 25 mm. Dots and circles give quick references for these two sizes. In addition, a scale is incorporated.

The microscope must be calibrated when ordering this graticule, such that the circle must equate to 1 mm on the microscope stage.

Reference

• The United State Pharmaceutical Conventions, Inc. Pharmaceutical Forum. Vol. 19 No 6.

Pattern	Description	Dia	Cat #	Qty	Price
G57	Pharmaceutical PSA Pattern. Glass Sandwich.	19 mm	68091-19	each	282.00	Add to Cart
		20.4 mm	68091-20.4	each	282.00	Add to Cart
		21 mm	68091-21	each	282.00	Add to Cart
		23 mm	68091-23	each	282.00	Add to Cart
		24.5 mm	68091-24.5	each	290.00	Add to Cart
		25 mm	68091-25	each	290.00	Add to Cart
		26 mm	68091-26	each	290.00	Add to Cart
		27 mm	68091-27	each	300.00	Add to Cart

Counting Pattern - NG14

Simple counting for geological and soil analysis.

Reference

• L.G. Briarty. "Stereology: Methods for Quantitative Light and Electron Microscopy." Sci. Prog. Oxf. 1975 62; 1-32.

Pattern	Description	Dia	Cat #	Qty	Price
NG14	Counting pattern for soil analysis. 10 mm square. Surface chrome image.	16 mm	68092-16	each	250.00	Add to Cart
		19 mm	68092-19	each	232.00	Add to Cart
		21 mm	68092-21	each	232.00	Add to Cart

Lennox Grain Analysis - NG21

Pattern	Description	Dia	Cat #	Qty	Price
NG21	Lennox for Grain analysis.	16 mm	68004-16	each	266.00	Add to Cart
		19 mm	68004-19	each	266.00	Add to Cart
		21 mm	68004-21	each	266.00	Add to Cart
		23 mm	68004-23	each	266.00	Add to Cart
		Custom	68004-50	each	356.00	Add to Cart

Kotter Pattern - G48

For analysis of bitumous coal and anthracite.

Reference

• I.S.O. 7404-4: 1988 (E). Methods for Analysis of Bitumous Coal and Anthracite. Part 4 and Methods of Determining Microlithotype Composition.

Normally used with 20x objective = calibration factor of 1. For use with 40x objective, specify calibration factor of 2. For 50x specify 2.5. For other objective magnifications, the reticle will need to be custom made.

Pattern	Description	Dia	Cat #	Qty	Price
G48	Kotter Pattern for bitumous coal and anthracite analysis.	16 mm	67990-16	each	260.00	Add to Cart
		19 mm	67990-19	each	260.00	Add to Cart
		20.4 mm	67990-20.4	each	260.00	Add to Cart
		21 mm	67990-21	each	260.00	Add to Cart
		23 mm	67990-23	each	260.00	Add to Cart
		24 mm	67990-24	each	310.00	Add to Cart
		24.5 mm	67990-24.5	each	310.00	Add to Cart
		25 mm	67990-25	each	310.00	Add to Cart
		26 mm	67990-26	each	310.00	Add to Cart
		27 mm	67990-27	each	310.00	Add to Cart
		Custom	67990-50	each	350.00	Add to Cart

Zeiss Integrating Eyepiece Disc 100 Points - G47

Similar to G49, but extended to 100 points, which are indexed.

Pattern	Description	Dia	Cat #	Qty	Price
G47	Zeiss integrating eyepiece disc. Glass sandwich.	16 mm	68094-16	each	280.00	Add to Cart
		19 mm	68094-19	each	280.00	Add to Cart
		21 mm	68094-21	each	280.00	Add to Cart

Zeiss Integrating Disc 1 or Henning Reseau Pattern 25 Points - G49

Reference

• Zeiss Werkzeitschrft. No 30, page 80.

Pattern	Description	Dia	Cat #	Qty	Price
G49	Henning Resseau pattern (Zeiss integrating disc 1). Glass sandwich.	16 mm	68093-16	each	274.00	Add to Cart
		19 mm	68093-19	each	274.00	Add to Cart
		21 mm	68093-21	each	274.00	Add to Cart

Integrating EyePiece - G50

Pattern	Description	Dia	Cat #	Qty	Price
G50	Integrated EyePiece (Simplified).	19 mm	68005-19	each	242.00	Add to Cart
		21 mm	68005-21	each	242.00	Add to Cart
		23 mm	68005-23	each	256.00	Add to Cart
		24 mm	68005-24	each	282.00	Add to Cart
		Custom	68005-50	each	338.00	Add to Cart

G. Stereology

In this simplest form, sterology is the science where information about a three dimensional object is obtained from only a two-dimensional section of that structure.

Measurements are usually made with these graticules in the following manner:

1. An adequate representation of sections of a specimen is obtained.
2. The graticule is superimposed upon the specimen (or micrograph/projected image of the section).
3. Finally, the interaction between the superimposed graticule and the test sections are recorded.

An overall introduction is given by: L.B. Brianrty. "Stereology: Methods for Quantitative Light and Electron Microscopy."

The Mertz Graticule (36 Points) - NGM1

Used to estimate the three-dimensional surface areas or the surface density of a component in a given volume, when the component does not have a random orientation. It comprises a test system with parallel curved lines used for measuring the intersection of points.

Reference

• W.A. Mertz. "Mikroskopic" Vol. 22 1967 pp 132-142.

Pattern	Description	Dia	Cat #	Qty	Price
NGM1	Mertz for Stereology. Surface chrome image.	16 mm	68035-16	each	244.00	Add to Cart
		19 mm	68035-19	each	244.00	Add to Cart
		21 mm	68035-21	each	244.00	Add to Cart

Weibel 1 - NGW1

Consists of 15 lines of equal length connecting the verticals of a regular hexagonal point network.

Reference

• E.R.Weibel Lab. Invest. Vol. 22 pp 131-152. "Principal and Methods for the Morphometric Study of the Lung and other Organs."

Pattern	Description	Dia	Cat #	Qty	Price
NGW1	Weibel Type 1 for Stereology. Surface chrome image.	16 mm	68022-16	each	248.00	Add to Cart
		19 mm	68022-19	each	248.00	Add to Cart
		21 mm	68022-21	each	248.00	Add to Cart

Weibel 2 - NGW2

Used when making a surface to volume ratio of a structure per mass unit. This graticule consists of a number of short lines with interruptions as long as the lines. Basically, the number of intersections falling over the short lines is counted and the number of endpoints falling on the end of the structure is determined.

Reference

• E.R. Weibel, Journal of Microscopy Vol. 95. pp 373-378. Current Capabilities and Limitations of Available Sterrological Technique, point counting method.

Pattern	Description	Dia	Cat #	Qty	Price
NGW2	Weibel Type 2 for Stereology. Surface chrome image.	16 mm	68025-16	each	248.00	Add to Cart
		19 mm	68025-19	each	248.00	Add to Cart
		21 mm	68025-21	each	248.00	Add to Cart

Weibel 3 - GW3

Reference

• E.R. Weibel, G.S. Kishtler & W.F. Scherle. 1986. J. Cell Biology. 30, 23.

Pattern	Description	Dia	Cat #	Qty	Price
NGW3	Weibel Type 3 for Stereology. Glass sandwich.	16 mm	68023-16	each	248.00	Add to Cart
		19 mm	68023-19	each	248.00	Add to Cart
		21 mm	68023-21	each	248.00	Add to Cart

H. Metallurgy

Standard pattern discs for metallurgical stereometric analysis of grain size in polished metal sections.

Grain Sizing Pattern EN10247/ISO4976

For the determination of non-metallic inclusion content of steel.

NG60 meets EN 10247 and NG61 Meets ISO 4976. Both are scaled for use eith 10x objective magnification Pattern

Pattern	Description	Dia	Cat #	Qty	Price
NG60	Grain Sizing reticle to EN 10247.	21 mm	68007-21	each	262.00	Add to Cart
		23 mm	68007-23	each	262.00	Add to Cart
		24.5 mm	68007-24	each	306.00	Add to Cart
		25 mm	68007-25	each	306.00	Add to Cart
		26 mm	68007-26	each	306.00	Add to Cart
		27 mm	68007-27	each	306.00	Add to Cart
		Custom	68007-50	each	342.00	Add to Cart
NG61	Grain Sizing reticle to ISO 4967.	21 mm	68036-21	each	262.00	Add to Cart
		23 mm	68036-23	each	262.00	Add to Cart
		24.5 mm	68036-24	each	302.00	Add to Cart
		25 mm	68036-25	each	302.00	Add to Cart
		26 mm	68036-26	each	302.00	Add to Cart
		27 mm	68036-27	each	302.00	Add to Cart
		Custom	68036-50	each	340.00	Add to Cart

ASTM Austenite 1:1 Grain Sizing Disc - G41

Reference

• VDEH 1510-61.

Pattern	Description	Dia	Cat #	Qty	Price
G41	ASTM Grain Size Austenite. Glass sandwich.	19 mm	68065-19	each	290.00	Add to Cart
		21 mm	68065-21	each	290.00	Add to Cart

ASTM E112 Plate 1 Grain Sizing Disc - G42

Pattern	Description	Dia	Cat #	Qty	Price
G42	Grain Sizing (E112). Glass Sandwich.	19 mm	68066-19	each	270.00	Add to Cart
		21 mm	68066-21	each	270.00	Add to Cart

ASTM Carbide Grain Sizing Chart - G43

Pattern	Description	Dia	Cat #	Qty	Price
G43	ASTM Grain Sizing carbide. Glass sandwich.	19 mm	68067-19	each	270.00	Add to Cart
		21 mm	68067-21	each	270.00	Add to Cart

ASTM E45 - G44

Reference

• ASTM E45.

Pattern	Description	Dia	Cat #	Qty	Price
G44	ASTM E45, Root 2 sides. 7.1 mm square, 10 mm scale.	19 mm	68068-19	each	270.00	Add to Cart
		21 mm	68068-21	each	270.00	Add to Cart

ASTM E19-46 Grain Sizing Disc - G45

Pattern	Description	Dia	Cat #	Qty	Price
G45	ASTM Grain Sizing E19-46. Glass sandwich.	19 mm	68069-19	each	270.00	Add to Cart
		21 mm	68069-21	each	270.00	Add to Cart

ASTM E19-46 Grain Sizing Disc Root 2 - G46

Pattern	Description	Dia	Cat #	Qty	Price
G46	ASTM Grain sizing E19-46 Root 2 Glass sanwich	19 mm	68070-19	each	270.00	Add to Cart
		21 mm	68070-21	each	270.00	Add to Cart

Circular Grid ASTM 24 Points - G54

Reference

• ASTM G562.

Pattern	Description	Dia	Cat #	Qty	Price
G54	ASTM 24 Point Circular Grid. Glass sandwich.	16 mm	68084-16	each	280.00	Add to Cart
		19 mm	68084-19	each	280.00	Add to Cart
		21 mm	68084-21	each	280.00	Add to Cart

Square Grid ASTM 25 Points - G55

Reference

• ASTM G562.

Pattern	Description	Dia	Cat #	Qty	Price
G55	ASTM 25 Point Square Grid. Glass sandwich.	16 mm	68085-16	each	280.00	Add to Cart
		19 mm	68085-19	each	280.00	Add to Cart
		21 mm	68085-21	each	280.00	Add to Cart

Stage Graticules