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Electron Microscopy Sciences


Kirsch Notes, Technical Tips and Recommended Articles

Kirsch Notes

The "CliffsNotes"-like style of protocols and recipes for microscopy.

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Bio EM Processing – Start to Finish
Developing Film for TEM
Köhler Alignment for the Optical Light Microscope (OLM)
Three Techniques for Retrieving Thin Sections
Critical Point Drying (CPD) with the E3100
Critical Point Drying (CPD) with the K850
How to Thin Section TEM Samples
Biological Processing Steps for TEM
SEM Biological HMDS Processing
Thick Sectioning for TEM Using the RMC Power Tome PC
Preparation of Post Staining Solutions
Preparing Karnovsky's Fixative From Scratch
Thick Sectioning Using the Leica UC7 Ultramicrotome
Embedding Samples
Guidelines for Qualitative X-ray Analysis
Block Trimming for TEM
Preparing Osmium Tetroxide from Crystalline
Mixing Epoxy Resins
Providing Solutions
Dehydrants for Electron Microscopy
General Lab Safety
SEM Specimen Mounting
Fixatives for Electron Microscopy
Buffers for Electron Microscopy
TEM Embedding Resins
TEM Imaging Issues and Solutions
Challenges with Resins

Technical Tips and Recommended Article Index

An array of articles and technical tips located throughout our site assembled in one place.

Use of Acetonitrile
Mounting of Powders, Granules, and Fibers
DEPEX: An improved Mounting Media
Purification of Alcian Blue
Use of Accelerators for Epoxy Resins
Tips for Butvar B-98 Support Films
Preservation of Phospholipids
The Use of Polyethylene Glycol
The Use of Flexibilizers and Plasticizers
Stability of Staining Solutions
The Use of DMP (2,2-Dimethoxy Propane)
An Epon-812 Replacement
Re-Embedding of Tissue Poorly Embedded
The Fixative for Use in Fixing Cornea
The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer
Preparation of Gelatin Adhesive
Preparation of Glycol Methacrylate
Hematoxylin Staining Tips
Elimination of Section Contamination During Staining
Lead Staining
Common Lead Stock Solutions
Handling of Lead Solutions
A Stable Lead Staining Solution
Embedding Resin Stain Permability
Flat Embedding with LR-White
A Methylene Blue-Azure II Stain
Storage of Epoxy Resins
Paraformaldehyde Static Jumps
Re-Embedding of Paraffin Embedded Specimens in Resin
Making and Air Tight Container for Osmium Tetroxide
Neutralization and Disposal of Osmium Tetroxide
Flattening Semithin Sections of Epoxy-Embedded Tissues
Temperature Conversions
Improving the Adhesion of Sections Filmless Grids
A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissue
Avoiding Contamination of Ultra-Thin Sections
Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections
ImmunoGold Frequently Asked Questions
The Dot Spot Test
Ultrasmall Gold Conjugates and Silver Enhancements
Optimized Immunolabeling
Epoxy Resins: Problems, Causes, and Solutions
Storage of Embedding Resin Mixtures
Preparation of Embedding Mixtures
Adherence of LR-White Sections to Glass Slides for Immunogold Labeling
LR White General Tips
Improved Polymerization of Acrylic Resins
Low Viscosity Kit Formulation with ERL 4221
Quetol and the Addition of Water
Rapid Techniques for Specimen Preparation
Grids: Shiny Side vs Dull Side
Grids: Removing a Charge from the Surface
On Grid Enhancement
Reaction of Ni and Cu Grids
Picking up of Carbon Film
A Simple Method for Handling Grids
Section Pick-up; The Perfect Loop
Inspecting the SEM While in Operation
A Method for Slide Embedding
Dental Wax to Make Trufs
Surgical Gloves: A Safety Tip
Latex Gloves: Allergy and Alternatives
Permanox Plastic; Non Fluorescent
Poly-L-Lysine Coating of Slides
Certified Dyes and Stains: An Application Guide
Animal Tissue Techniques
Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen
Bone Marrow Biopsies
The Mounting of Specimens for SEM with the Common Glue Gun
Cryo Sectioning and the Use of Diamond Knives
Darkroom Safelights
New 100% Ozone Safe Dusters
Increase Stability and Shelf Life of Chemicals
Elimination of Wrinkles in Semi-Thin Sections: A Vacuum Drying Technique
Safety in the Lab: Publications
Ultra Small ImmunoGold Reagents Application Instructions
Conventional ImmunoGold Reagents Application Instructions
Why Ultra Small Gold Conjugates
Blocking Solutions Application Instructions
Incubation Solution Additive AURION BSA-c™
Controlling Background in Immunogold Labeling
Application Instructions for Solutions with BSA, CWFS, and Normal Sera
R-Gent Silver Enhancement Reagents Application Instructions
Gold Tracers Application Instructions
Enhancement Conditioning Solution
Reactivity of Protein A and Protein G
Pre-embedding Immunogold Procedures
Double Labeling with two Ultra Small Reagents
Cryo Preparation using C-flat
Coverslip Treatment Test
Achieving Maximum Contrast with Stain
Elimination of Section Contamination During Staining
Stability of Special Staining Solutions
Preparation of Formvar Film-coated Grids
A Fool-Proof Method for Mounting Serial Sections on Single Hole Grids