Technical

Kirsch Notes, Technical Tips and Recommended Articles

Kirsch Notes

The "CliffsNotes"-like style of protocols and recipes for microscopy.

Protocols

Bio EM Processing – Start to Finish

Köhler Alignment for the Optical Light Microscope (OLM)

Three Techniques for Retrieving Thin Sections

Critical Point Drying (CPD) with the EMS 3100

Critical Point Drying (CPD) with the EMS Q850

How to Thin Section TEM Samples

Biological Processing Steps for TEM

SEM Biological HMDS Processing

Thick Sectioning for TEM Using the RMC Power Tome PC

Solutions

Preparation of Post Staining Solutions

Preparing Karnovsky's Fixative From Scratch

Thick Sectioning Using the Leica UC7 Ultramicrotome

Embedding Samples

Guidelines for Qualitative X-ray Analysis

Block Trimming for TEM

Preparing Osmium Tetroxide from Crystalline

Mixing Epoxy Resins

Developing Film for TEM

Uranyless

Providing Solutions

Dehydrants for Electron Microscopy

SEM Specimen Mounting

Fixatives for Electron Microscopy

Buffers for Electron Microscopy

TEM Embedding Resins

TEM Imaging Issues and Solutions

Challenges with Resins

General Lab Safety

Technical Tips and Recommended Article Index

An array of articles and technical tips located throughout our site assembled in one place.

Use of Acetonitrile

Mounting of Powders, Granules, and Fibers

DEPEX: An improved Mounting Media

Purification of Alcian Blue

Use of Accelerators for Epoxy Resins

Tips for Butvar B-98 Support Films

Preservation of Phospholipids

The Use of Polyethylene Glycol; Carbowax

The Use of Flexibilizers and Plasticizers

Stability of Staining Solutions

The Use of DMP (2,2-Dimethoxy Propane)

Cryo-Protectants

An Epon-812 Replacement

Re-Embedding of Tissue Poorly Embedded

The Fixative for Use in Fixing Cornea

The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer

Preparation of Gelatin Adhesive

Preparation of Glycol Methacrylate

Hematoxylin Staining Tips

Elimination of Section Contamination During Staining

Lead Staining

Common Lead Stock Solutions

Handling of Lead Solutions

A Stable Lead Staining Solution

Embedding Resin Stain Permability

Flat Embedding with LR-White

A Methylene Blue-Azure II Stain

Storage of Epoxy Resins

Paraformaldehyde Static Jumps

Re-Embedding of Paraffin Embedded Specimens in Resin

Making and Air Tight Container for Osmium Tetroxide

Neutralization and Disposal of Osmium Tetroxide

Flattening Semithin Sections of Epoxy-Embedded Tissues

Temperature Conversions

Improving the Adhesion of Sections Filmless Grids

A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissue

Avoiding Contamination of Ultra-Thin Sections

Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections

ImmunoGold Frequently Asked Questions

The Dot Spot Test

Ultrasmall Gold Conjugates and Silver Enhancements

Optimized Immunolabeling

Epoxy Resins: Problems, Causes, and Solutions

Storage of Embedding Resin Mixtures

Preparation of Embedding Mixtures

DMP-30 and DMAE VS. BDMA

Adherence of LR-White Sections to Glass Slides for Immunogold Labeling

LR White General Tips

Improved Polymerization of Acrylic Resins

Low Viscosity Kit Formulation with ERL 4221

Quetol and the Addition of Water

Rapid Techniques for Specimen Preparation

Grids: Shiny Side vs Dull Side

Grids: Removing a Charge from the Surface

On Grid Enhancement

Reaction of Ni and Cu Grids

Picking up of Carbon Film

A Simple Method for Handling Grids

Section Pick-up; The Perfect Loop

Inspecting the SEM While in Operation

A Method for Slide Embedding

Dental Wax to Make Trufs

Surgical Gloves: A Safety Tip

Latex Gloves: Allergy and Alternatives

Permanox Plastic; Non Fluorescent

Pap-Pen

Poly-L-Lysine Coating of Slides

Certified Dyes and Stains: An Application Guide

Animal Tissue Techniques

Parafin and Plastic Techniques for Preparation of Bone Marrow Specimen

Bone Marrow Biopsies

The Mounting of Specimens for SEM with the Common Glue Gun

Cryo Sectioning and the Use of Diamond Knives

Darkroom Safelights

New 100% Ozone Safe Dusters

Increase Stability and Shelf Life of Chemicals

Elimination of Wrinkles in Semi-Thin Sections: A Vacuum Drying Technique

Safety in the Lab: Publications

Ultra Small ImmunoGold Reagents Application Instructions

Conventional ImmunoGold Reagents Application Instructions

Why Ultra Small Gold Conjugates

Blocking Solutions Application Instructions

Incubation Solution Additive AURION BSA-c™

Controlling Background in Immunogold Labeling

Application Instructions for Solutions with BSA, CWFS, and Normal Sera

R-Gent Silver Enhancement Reagents Application Instructions

Col-Aurion

Gold Tracers Application Instructions

Reactivity of Protein A and Protein G

Pre-embedding Immunogold Procedures

Double Labeling with two Ultra Small Reagents

Cryo Preparation using C-flat

Coverslip Treatment Test

Achieving Maximum Contrast with Stain

Elimination of Section Contamination During Staining

Stability of Special Staining Solutions

Preparation of Formvar Film-coated Grids

A Fool-Proof Method for Mounting Serial Sections on Single Hole Grids

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Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.