Certificates of Analysis

Ready To Use Prepared Buffers

All of our ready-to-use buffers are:

• Conveniently packaged.
• Prepared fresh for each order.
• Economical and time-saving.
• Desired pH should be specified when ordering.

The buffer is a vehicle for the fixative and requires certain properties:

1. To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
2. The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.

Acetate Buffer

Acetic Acid and Sodium Acetate, 0.2M. pH range 3.6-5.6.

2-8°C	11480	Acetate Buffer	225 ml	19.00	Add to cart
2-8°C	11482	Acetate Buffer	500 ml	23.50	Add to cart
2-8°C	11482-1L	Acetate Buffer	1 L	46.00	Add to Cart

Acetate Buffer

2-8°C	11482-42	Acetate Buffer, 0.2M, pH 4.2		500 ml	23.50	Add to Cart
2-8°C	11482-42-1L	Acetate Buffer, 0.2M, pH 4.2		1 L	46.00	Add to Cart
2-8°C	11482-50	Acetate Buffer, 0.2M, pH 5.0		500 ml	23.50	Add to Cart
2-8°C	11482-50-1L	Acetate Buffer, 0.2M, pH 5.0		1 L	46.00	Add to Cart
2-8°C	11482-56	Acetate Buffer, 0.2M, pH 5.6		500 ml	23.50	Add to Cart
2-8°C	11482-56-1L	Acetate Buffer, 0.2M, pH 5.6		1 L	46.00	Add to Cart

Antibody Diluent

Buffer for diluting your primary and secondary antibodies, especially if stored for extended time, even at -20°C in glycerol, or refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimized, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.

When used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.

For especially "trouble"-giving antibodies, as well as for in situ PCR applications, this dilutent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.

4-8°C	62713	Antibody Diluent		50 ml	172.00	Add to Cart
4-8°C	62714	Antibody Diluent		125 ml	375.00	Add to Cart
4-8°C	62715	Antibody Diluent		500 ml	1,400.00	Add to Cart
4-8°C	62713-01	Antibody Diluent for Frozen Sections		50 ml	172.00	Add to Cart
4-8°C	62714-01	Antibody Diluent for Frozen Sections		125 ml	375.00	Add to Cart
4-8°C	62715-01	Antibody Diluent for Frozen Sections		500 ml	1,400.00	Add to Cart

Antibody Dilution Buffer with Immunoglobulin-Free BSA

This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.

2-8°C	25886-01	Antibody Dilution Buffer, free BSA	100 ml	66.00	Add to Cart
2-8°C	25886-05	Antibody Dilution Buffer, free BSA	500 ml	178.00	Add to Cart
	1227SK	Antigen Preserve Solution VII	1L	156.00	Add to Cart

Borate Buffer

RT	11455-78	Borate Buffer, pH 7.8		500 ml	24.00	Add to Cart
RT	11455-82	Borate Buffer, pH 8.2		500 ml	35.00	Add to Cart
RT	11455-90	Borate Buffer, pH 9.0		500 ml	35.00	Add to Cart

Citrate Buffer

Citric Acid and Sodium Citrate, 0.1M. pH range 4.6-6.2.

2-8°C	11490	Citrate Buffer, 0.1M	225 ml	19.00	Add to Cart
2-8°C	11492	Citrate Buffer, 0.1M	500 ml	23.50	Add to Cart
2-8°C	11492-1L	Citrate Buffer, 0.1M	1 L	42.00	Add to Cart

Citrate Buffer

Citric Acid and Sodium Citrate 0.2M.

2-8°C	11492-04	Citrate Buffer, 0.2M, pH 4.0		1 L	42.50	Add to Cart
2-8°C	11492-06	Citrate Buffer, 0.2M, pH 6.0		1 L	42.50	Add to Cart

Citrate Buffer pH 6.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution:Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

2-8°C	64142-07	Citrate Buffer pH 6.0	100 ml	78.00	Add to Cart
2-8°C	64142-08	Citrate Buffer pH 6.0	1000 ml	130.00	Add to Cart
2-8°C	64142-08-1G	Citrate Buffer pH 6.0	1Gal.	325.00	Add to Cart

Dulbecco's Phosphate Buffer Saline – D-PBS

All of our Dulbecco's Phosphate Buffer Saline Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1x strength.

Ingredients	F.W.	Formula I	Formula II	Formula III
KCl	74.56	X	X	X
NaCl	58.44	X	X	X
Na2HPO4·7H2O	268.07	X	X	X
KH2PO4	136.09	X	X	X
CaCl2·2H2O	147.02	X	X	X
MgCl2·6H2O	203.31	X	X	X
Phenol Red		----	----	0.2 g/L

RT	11772-05	D-PBS, Formula I, 0.15M		500 ml	26.00	Add to Cart
RT	11772-10	D-PBS, Formula I, 0.15M		1 L	31.00	Add to Cart
RT	11772-10-1G	D-PBS, Formula I, 0.15M		1 Gal.	75.00	Add to Cart
RT	11773-05	D-PBS, Formula II, 0.19M		500 ml	26.00	Add to Cart
RT	11773-10	D-PBS, Formula II, 0.19M		1 L	32.00	Add to Cart
RT	11773-10-1G	D-PBS, Formula II, 0.19M		1 Gal.	76.00	Add to Cart
RT	11774-05	D-PBS, Formula III, 0.15M		500 ml	31.00	Add to Cart
RT	11774-10	D-PBS, Formula III, 0.15M		1 L	35.00	Add to Cart
RT	11774-10-1G	D-PBS, Formula III, 0.15M		1 Gal.	78.00	Add to Cart

EARLE's Balanced Salt Solutions - EBSS

All of our Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1x strength.

Ingredients	F.W.	Formula I	Formula II	Formula III
KCl	74.56	X	X	X
NaCl	58.44	X	X	X
NaH2PO4·H2O	137.99	X	X	X
D-Glusose	180.16	1g/L	X	1g/L
MgSO4·7H2O	246.48	X	X	X
CaCl2,·2H2O	147.02	X	X	X
NaHCO3	84.01	X	X	X
Phenol Red		----	0.015g/L	0.050g/L

RT	11765-05	EBSS, Formula I, 0.157M		500 ml	23.00	Add to Cart
RT	11765-10	EBSS, Formula I, 0.157M		1 L	27.00	Add to Cart
RT	11765-10-1G	EBSS, Formula I, 0.157M		1 Gal.	82.00	Add to Cart
RT	11766-05	EBSS, Formula II, 0.152M		500 ml	22.00	Add to Cart
RT	11766-10	EBSS, Formula II, 0.152M		1 L	27.00	Add to Cart
RT	11766-10-1G	EBSS, Formula II, 0.152M		1 Gal.	82.00	Add to Cart
RT	11767-05	EBSS, Formula III, 0.157M		500 ml	22.00	Add to Cart
RT	11767-10	EBSS, Formula III, 0.157M		1 L	27.00	Add to Cart
RT	11767-10-1G	EBSS, Formula III, 0.157M		1 Gal.	82.00	Add to Cart

EDTA Buffer pH 8.5 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C.DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 8.5 antigen retriever solution.
Preparation of Working Solution:Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

2-8°C	64142-09	EDTA Buffer pH 8.5	100 ml	78.00	Add to Cart
2-8°C	64142-10	EDTA Buffer pH 8.5	1000 ml	130.00	Add to Cart
2-8°C	62142-10-1G	EDTA Buffer pH 8.5	1 Gal.	300.00	Add to Cart

Hepes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

Hepes Buffer Solution

0.2M
pH range: 7.0-8.0

0-5°C

11494

Hepes Buffer Solution

500 ml

23.00

Add to Cart

HRP Conjugate Diluent

Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay preparing ready-to-use conjugate solutions in advance and store them in refrigerator without loss of activity.

4-8°C	62716	HRP Conjugate Diluent	50 ml	172.00	Add to Cart
4-8°C	62717	HRP Conjugate Diluent	125 ml	395.00	Add to Cart
4-8°C	62718	HRP Conjugate Diluent	500 ml	1,410.00	Add to Cart

Michel's Buffer

RT	18041-05	Michel's Buffer	500 ml	23.00	Add to Cart
RT	18041-10	Michel's Buffer	1 L	30.00	Add to Cart
RT	18041-10-1G	Michel's Buffer	1 Gal.	95.00	Add to Cart
RT	18041-10-5G	Michel's Buffer	5 Gal.	450.00	Add to Cart

Millonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH₂PO₄xH₂O)
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature

The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

2-8°C	11582-05	Millonig's Phosphate Buffer 0.2M	500 ml	23.50	Add to Cart
2-8°C	11582-10	Millonig's Phosphate Buffer 0.2M	1 L	33.00	Add to Cart
2-8°C	11582-10-1G	Millonig's Phosphate Buffer 0.2M	1 Gal.	120.00	Add to Cart
2-8°C	11582-10-5G	Millonig's Phosphate Buffer 0.2M	5 Gal.	525.00	Add to Cart

Mops, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid)
pH range 6.5-7.9

0-5°C

18900

Mops, Biological Buffer

100g

74.00

Add to Cart

PHEM Buffer

EMS is proud to announce and release of our newest Buffer for EM PHEM.

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies.  Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

References:

1. Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
2. Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers

Academic Editor: James Reimer, published online March 31, 2016

2-8°C	11162	PHEM Buffer, 0.1M	225ml	45.00	Add to Cart
2-8°C	11163	PHEM Buffer, 0.1M	500ml	76.00	Add to Cart
2-8°C	11164	PHEM Buffer, 0.2M	225ml	50.00	Add to Cart
2-8°C	11165	PHEM Buffer, 0.2M	500ml	84.00	Add to Cart

Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

2-8°C	19340-60	Phosphate Buffer, pH 6.0		1 L	40.00	Add to Cart
2-8°C	19340-60-1G	Phosphate Buffer, pH 6.0		1 Gal.	104.00	Add to Cart
2-8°C	19340-65	Phosphate Buffer, pH 6.5		1 L	40.00	Add to Cart
2-8°C	19340-65-1G	Phosphate Buffer, pH 6.5		1 Gal.	104.00	Add to Cart
2-8°C	19340-70	Phosphate Buffer, pH 7.0		1 L	40.00	Add to Cart
2-8°C	19340-70-1G	Phosphate Buffer, pH 7.0		1 Gal.	104.00	Add to Cart
2-8°C	19340-72	Phosphate Buffer, pH 7.2		1 L	40.00	Add to Cart
2-8°C	19340-72-1G	Phosphate Buffer, pH 7.2		1 Gal.	104.00	Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

Phosphate Buffer Saline (PBS)

PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na₂HPO₄, 0.24 g/L KH₂PO₄ and pH 7.4.

2-8°C	19342-10	Phosphate Buffered Saline, 10X		1 L	32.00	Add to Cart
2-8°C	19242-40	Phosphate Buffered Saline, 10X		1 gal	99.00	Add to Cart
2-8°C	19343-10	Phosphate Buffered Saline, 2X		1 L	28.00	Add to Cart
2-8°C	19343-40	Phosphate Buffered Saline, 2X		1 gal	88.00	Add to Cart
2-8°C	19344-10	Phosphate Buffered Saline, 1X		1 L	24.00	Add to Cart
2-8°C	19344-20	Phosphate Buffered Saline, 1X		1 gal	76.00	Add to Cart

Pipes, Good's Buffer Crystalline

Please see listing in Chemicals for Microscopy.

Pipes Buffer Solution, 0.3M

Aqueous PIPES solution adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.

0-5°C

11610

Pipes Buffer Solution, 0.3M

450 ml

51.00

Add to Cart

R-UNIVERSAL Epitope Recovery Buffer (10x stock)

Why the Universal Buffer?

Epitope recovery on formalin-fixed, paraffin embedded tissue sections, requires heat-induced treatment in buffer or, sometimes, proteolitic treatment of the deparaffinized tissue section. Which buffer to use, greatly depends on the exact antibody and the properties of the recognized epitope, therefore, one can find in literature and practice use of many buffers, including Citrate pH 3.4, Citrate 6.0, EDTA 8.0, Tris 9.0- 10.0, Tris-EDTA, etc.

Moreover, for individual antigens also the time of recovery in the individual buffer should be defined, as this may be different, and the treatment often destroys the epitope.

EMS offers a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups.

The buffer was extensively tested in pathology Departments in United Kingdom, and has shown excellent results when used with different antibodies, including those that normally require for successful staining treatment only in Low, or only High pH buffers, or require protolithic treatment.

The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series).

Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections.

Properties

Clear, non-toxic solution.

Presentation

R-Universal Buffer is supplied as 10x concentrate. For epitope recovery dilute 1 part of stock with 9 parts of deionized water.

Application

For epitope recovery dilute 1 part of stock with 9 parts of deionized water.

Stability and Storage

The preparation is stable for 1 year when stored unopened at +40°C. Every lot is issued with a certificate indicating the expiry date.

After opening, store at +40°C in the refrigerator and use within 6 months.

Certification

Each lot is certified for compliance to specifications. The product is produced under DIN EN ISO 9001 :2008 Quality Management system for the products in Immunoassay Development and Measurement, Products for Bioanalytics and lmmunoassays.

Ordering Information:

62719-10	R-UNIVERSAL Epitope Recovery Buffer (10x stock)	125 ml	149.00	Add to Cart
62719-20	R-UNIVERSAL Epitope Recovery Buffer (10x stock)	500 ml	240.00	Add to Cart

Section Block

A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum-based blocking solutions there is no interaction of specific antibody and blocking protein itself. This is not comparable to other commercially available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.

4-8°C	62710	Section Block	50 ml	162.00	Add to Cart
4-8°C	62711	Section Block	125 ml	280.00	Add to Cart
4-8°C	62712	Section Block	500 ml	1,200.00	Add to Cart

Sodium Cacodylate Buffer

Prepared from Sodium Cacodylate Trihydrate (F.W. 214.02)

2-8°C	11650	Sodium Cacodylate Buffer, 0.2M, pH 7.4		225 ml	31.00	Add to Cart
2-8°C	11652	Sodium Cacodylate Buffer, 0.2M, pH 7.4		500 ml	44.00	Add to Cart
2-8°C	11653	Sodium Cacodylate Buffer, 0.2M, pH 7.4		1 L	86.00	Add to Cart
2-8°C	11653-1G	Sodium Cacodylate Buffer, 0.2M, pH 7.4		1 Gal.	217.00	Add to Cart
2-8°C	11654	Sodium Cacodylate Buffer, 0.4M, pH 7.2		500 ml	64.00	Add to Cart
2-8°C	11655	Sodium Cacodylate Buffer, 0.4M, pH 7.2		1 L	114.00	Add to Cart
2-8°C	11655-1G	Sodium Cacodylate Buffer, 0.4M, pH 7.2		1 Gal.	217.00	Add to Cart

Sorensen's Phosphate Buffer

(Phosphate Mixed Sodium Salts)
Sorensen, see Dawson et. al, (1969)
Sodium Phosphate, dibasic (Na₂HPO₄xH₂O)
Sodium Phosphate, monobasic (NaH₂PO₄xH₂O)
0.2M, pH range 5.8 - 8.0
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 3 months

The osmolarity of a 0.1 M at a pH 7.2 is 226 mosmols; with the addition of 0.18 M of sucrose to the solution, the osmolarity raises to 425 mosmols.
Standard pH 7.2; 0.2M is supplied. Otherwise, specify pH when ordering.

2-8°C	11600-05	Sorensens Phosphate Buffer, 0.2M, pH 7.2		500 ml	24.00	Add to Cart
2-8°C	11600-10	Sorensens Phosphate Buffer, 0.2M, pH 7.2		1 L	33.00	Add to Cart
2-8°C	11600-10-4L	Sorensens Phosphate Buffer, 0.2M, pH 7.2		4 L	110.00	Add to Cart
2-8°C	11600-10-5G	Sorensens Phosphate Buffer, 0.2M, pH 7.2		5 Gal.	289.00	Add to Cart
2-8°C	11600-40	Sorensens Phosphate Buffer, 0.2M, pH 7.2		4 L	111.00	Add to Cart
2-8°C	11600-40-5G	Sorensens Phosphate Buffer, 0.2M, pH 7.2		5 Gal.	289.00	Add to Cart
2-8°C	11601-10	Sorensens Phosphate Buffer, 0.2M, pH 7.4		1 L	34.00	Add to Cart
2-8°C	11601-40	Sorensens Phosphate Buffer, 0.2M, pH 7.4		4 L	111.00	Add to Cart
2-8°C	11601-40-5G	Sorensens Phosphate Buffer, 0.2M, pH 7.4		5 Gal.	289.00	Add to Cart
2-8°C	11682-05	Sorensen's Phosphate Buffer, 0.15M		500 ml	27.00	Add to Cart
2-8°C	11682-10	Sorensen's Phosphate Buffer, 0.15M		1 L	34.00	Add to Cart
2-8°C	11682-10-4L	Sorensen's Phosphate Buffer, 0.15M		4 L	115.00	Add to Cart
2-8°C	11682-10-5G	Sorensen's Phosphate Buffer, 0.15M		5 Gal.	296.00	Add to Cart

Sym-Collidine Buffer Kit

2,4,6-Trimethylpyridine
N:C(CH₃)CH:C(CH₃)CH:CCH₃   CAS #108-75-8   F.W. 121.18
Bennett & Luft., Biophys & Biochem. Cytol., 6,113 (1959).

A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity. The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200 ml. Preparation Instructions: Pour 9 ml of 2N HCl; add 5.34 ml of Sym-Collidine, mix well and add distilled water up to 200 ml for a pH 7.4 solution.

Set consists of:

• 1 amp 5.34 ml Sym Collidine
• 1 amp 9.0 ml of 2N HCl

0-5°C	11500	Sym-Collidine Buffer Kit	Kit	33.00	Add to Cart
0-5°C	11510	Sym-Collidine Buffer	5 kit/bx	112.00	Add to Cart

Sym-Collidine, Reagent

2,4,6-Collidine; 2,4,6-Trimethylpyridine
(C₅H₂N)(CH₃)₃   CAS #108-75-8   F.W. 121.18
Assay: 99% Specific Gravity: 0.9170

RT

11520

Sym-Collidine, Reagent

100 ml

192.00

Add to Cart

Tris, (hydroxymethyl) aminomethane, Crystalline

Please see listing in Chemicals for Microscopy.

Tris, (hydroxymethyl) aminomethane maleate

Tris Maleate Buffer 0.2M pH range 6.4-8.0

2-8°C	11730	Tris Maleate Buffer 0.2M pH range 6.4-8.0	250 ml	24.00	Add to Cart
2-8°C	11740	Tris Maleate Buffer 0.2M pH range 6.4-8.0	500 ml	32.00	Add to Cart
2-8°C	11740-1L	Tris Maleate Buffer 0.2M pH range 6.4-8.0	1 L	50.00	Add to Cart
2-8°C	11740-1G	Tris Maleate Buffer 0.2M pH range 6.4-8.0	1 Gal.	175.00	Add to Cart

Tris Buffer 0.2M pH 8

RT	11730-05	Tris Buffer 0.2M pH 8.0	250 ml	24.00	Add to Cart
RT	11730-06	Tris Buffer 0.2M pH 8.0	500 ml	32.00	Add to Cart
RT	11730-06-1L	Tris Buffer 0.2M pH 8.0	1 L	50.00	Add to Cart
RT	11730-06-1G	Tris Buffer 0.2M pH 8.0	1 Gal.	175.00	Add to Cart

Tris Buffered Saline pH 7.4

2-8°C	11730-08	Tris Buffered Saline pH 7.4	250 ml	24.00	Add to Cart
2-8°C	11730-09	Tris Buffered Saline pH 7.4	500 ml	32.00	Add to Cart
2-8°C	11730-09-1L	Tris Buffered Saline pH 7.4	1 L	50.00	Add to Cart
2-8°C	11730-09-1G	Tris Buffered Saline pH 7.4	1 Gal.	175.00	Add to Cart

Tris HCL Buffer 1M pH 6-8

2-8°C	11730-12	Tris HCL Buffer 1M pH range 6-8	250 ml	24.00	Add to Cart
2-8°C	11730-15	Tris HCL Buffer 1M pH range 6-8	500 ml	32.00	Add to Cart
2-8°C	11730-15-1L	Tris HCL Buffer 1M pH range 6-8	1 L	50.00	Add to Cart
2-8°C	11730-15-1G	Tris HCL Buffer 1M pH range 6-8	1 Gal.	175.00	Add to Cart

Tris HCL Buffer pH range 7.2 - 9.0

2-8°C	11730-20	Tris HCL Buffer pH range 7.2-9.0	250 ml	24.00	Add to Cart
2-8°C	11730-25	Tris HCL Buffer pH range 7.2-9.0	500 ml	32.00	Add to Cart
2-8°C	11730-25-1L	Tris HCL Buffer pH range 7.2-9.0	1 L	50.00	Add to Cart
2-8°C	11730-25-1G	Tris HCL Buffer pH range 7.2-9.0	1 Gal.	175.00	Add to Cart

Tris Buffer pH 10.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.

2-8°C	64142-11	Tris Buffer pH 10.0	100 ml	78.00	Add to Cart
2-8°C	64142-12	Tris Buffer pH 10.0	1000 ml	130.00	Add to Cart
2-8°C	64142-12-1G	Tris Buffer pH 10.0	1 Gal.	350.00	Add to Cart

Universal Immunoassay Buffer™, 10x

Immunoassay buffer is used as a 1x in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.

This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: 10x Buffer

Application: Dilute this 10x buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.

Storage: Room temperature

RT	25880-01	Universal Immunoassay Buffer, 10x	100 ml	60.00	Add to Cart
RT	25880-10	Universal Immunoassay Buffer, 10x	1 L	162.00	Add to Cart

Universal Antibody Dilution Buffer™

This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.

Reagent Supplied: Ready to use buffer

Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.

Storage: 2–8°C. Diluted antibodies can be stored at 2–8°C for several weeks, depending upon the stability of antibodies.

2-8°C	25885-01	Universal Antibody Dilution Buffer	100 ml	66.00	Add to Cart
2-8°C	25885-05	Universal Antibody Dilution Buffer	500 ml	176.00	Add to Cart

Certificates of Analysis

Cacodylic to Durcupan