Ready To Use Prepared Buffers
All of our ready-to-use buffers are:
- Conveniently packaged.
- Prepared fresh for each order.
- Economical and time-saving.
- Desired pH should be specified when ordering.
The buffer is a vehicle for the fixative and requires certain properties:
- To maintain constant pH during the time of the fixation. The pH of the fixative in EM is adjusted using the buffer system chosen; e.g. range 6.5-8.0.
- The ionic constitution to prevent the extraction of cellular components, or the deposition of precipitates of fixative chemicals.

Acetate Buffer
Acetic Acid and Sodium Acetate
0.2M
pH range 3.6-5.6
0-5°C | 11480 | Acetate Buffer | 225 ml | 17.00 | Add to cart |
0-5°C | 11482 | Acetate Buffer | 500 ml | 22.00 | Add to cart |
Acetate Buffer
RT | 11482-42 | Acetate Buffer, 0.2M, pH 4.2 | ![]() |
500 ml | 22.00 | Add to Cart |
RT | 11482-50 | Acetate Buffer, 0.2M, pH 5.0 | ![]() |
500 ml | 22.00 | Add to Cart |
RT | 11482-56 | Acetate Buffer, 0.2M, pH 5.6 | ![]() |
500 ml | 22.00 | Add to Cart |


Antibody Diluent
Buffer for diluting your primary and secondary antibodies, especially if stored for extended time, even at -20°C in glycerol, or refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimized, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.
When used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.
For especially "trouble"-giving antibodies, as well as for in situ PCR applications, this dilutent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.
4-8°C | 62713 | Antibody Diluent | ![]() |
50 ml | 168.00 | Add to Cart |
4-8°C | 62714 | Antibody Diluent | 125 ml | 371.00 | Add to Cart | |
4-8°C | 62715 | Antibody Diluent | 500 ml | 1,395.00 | Add to Cart | |
4-8°C | 62713-01 | Antibody Diluent for Frozen Sections | 50 ml | 168.00 | Add to Cart | |
4-8°C | 62714-01 | Antibody Diluent for Frozen Sections | 125 ml | 371.00 | Add to Cart | |
4-8°C | 62715-01 | Antibody Diluent for Frozen Sections | 500 ml | 1,395.00 | Add to Cart |


Antibody Dilution Buffer with Immunoglobulin-Free BSA
This ready-to-use Antibody Dilution Buffer is designed for the dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer contains immunoglobulin free BSA and does not contain phosphate, sodium azide, or mercury preservatives. It can be used for the dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins. This buffer contains green food color.
Reagent Supplied: Ready to use buffer
Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.
Storage: 2-8°C. Diluted antibodies can be stored at 2-8°C for several weeks, depending upon the stability of antibodies.
2-8°C | 25886-01 | Antibody Dilution Buffer, free BSA | 100 ml | 60.00 | Add to Cart |
2-8°C | 25886-05 | Antibody Dilution Buffer, free BSA | 500 ml | 175.00 | Add to Cart |
1227SK | Antigen Preserve Solution VII | 1L | 152.00 | Add to Cart |
Borate Buffer
RT | 11455-78 | Borate Buffer, pH 7.8 | ![]() |
500 ml | 33.00 | Add to Cart |
RT | 11455-82 | Borate Buffer, pH 8.2 | ![]() |
500 ml | 33.00 | Add to Cart |
RT | 11455-90 | Borate Buffer, pH 9.0 | ![]() |
500 ml | 33.00 | Add to Cart |

Citrate Buffer
Citric acid and Sodium citrate 0.1M
pH range 4.6-6.2
0-5°C | 11490 | Citrate Buffer, 0.1M | 225 ml | 17.00 | Add to Cart |
0-5°C | 11492 | Citrate Buffer, 0.1M | 500 ml | 22.00 | Add to Cart |
Citrate Buffer
Citric acid and Sodium citrate 0.2M
0-5°C | 11492-04 | Citrate Buffer, 0.2M, pH 4.0 | ![]() |
1 L | 41.00 | Add to Cart |
0-5°C | 11492-06 | Citrate Buffer, 0.2M, pH 6.0 | ![]() |
1 L | 41.00 | Add to Cart |

Citrate Buffer pH 6.0 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution:Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C
4-8°C | 64142-07 | Citrate Buffer pH 6.0 | 100 ml | 75.00 | Add to Cart |
4-8°C | 64142-08 | Citrate Buffer pH 6.0 | 1000 ml | 145.00 | Add to Cart |
Dulbecco's Phosphate Buffer Saline – D-PBS
All of our Dullbecco's Phosphate Buffer Saline Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1x strength.
Ingredients | F.W. | Formula I | Formula II | Formula III |
KCl | 74.56 | X | X | X |
NaCl | 58.44 | X | X | X |
Na2HPO4·7H2O | 268.07 | X | X | X |
KH2PO4 | 136.09 | X | X | X |
CaCl2·2H2O | 147.02 | X | X | X |
MgCl2·6H2O | 203.31 | X | X | X |
Phenol Red | ---- | ---- | 0.2g/L |
RT | 11772-05 | D-PBS, Formula I, 0.15M | ![]() |
500 ml | 24.00 | Add to Cart |
RT | 11772-10 | D-PBS, Formula I, 0.15M | 1 L | 29.00 | Add to Cart | |
RT | 11773-05 | D-PBS, Formula II, 0.19M | ![]() |
500 ml | 24.00 | Add to Cart |
RT | 11773-10 | D-PBS, Formula II, 0.19M | 1 L | 30.00 | Add to Cart | |
RT | 11774-05 | D-PBS, Formula III, 0.15M | ![]() |
500 ml | 24.00 | Add to Cart |
RT | 11774-10 | D-PBS, Formula III, 0.15M | 1 L | 29.00 | Add to Cart |
EARLE's Balanced Salt Solutions - EBSS
All of our Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1x strength.
Ingredients | F.W. | Formula I | Formula II | Formula III |
KCl | 74.56 | X | X | X |
NaCl | 58.44 | X | X | X |
NaH2PO4·H2O | 137.99 | X | X | X |
D-Glusose | 180-16 | 1g/L | X | 1g/L |
MgSO4·7H2O | 246.48 | X | X | X |
CaCl2,·2H2O | 147.02 | X | X | X |
NaHCO3 | 84.01 | X | X | X |
Phenol Red | ---- | 0.015g/L | 0.050g/L |
RT | 11765-05 | EBSS, Formula I, 0.157M | ![]() |
500 ml | 21.00 | Add to Cart |
RT | 11765-10 | EBSS, Formula I, 0.157M | 1 L | 25.00 | Add to Cart | |
RT | 11766-05 | EBSS, Formula II, 0.152M | ![]() |
500 ml | 20.00 | Add to Cart |
RT | 11766-10 | EBSS, Formula II, 0.152M | 1 L | 25.00 | Add to Cart | |
RT | 11767-05 | EBSS, Formula III, 0.157M | ![]() |
500 ml | 20.00 | Add to Cart |
RT | 11767-10 | EBSS, Formula III, 0.157M | 1 L | 25.00 | Add to Cart |

EDTA Buffer pH 8.5 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C.DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 8.5 antigen retriever solution.
Preparation of Working Solution:Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C
4-8°C | 64142-09 | EDTA Buffer pH 8.5 | 100 ml | 75.00 | Add to Cart |
4-8°C | 64142-10 | EDTA Buffer pH 8.5 | 1000 ml | 125.00 | Add to Cart |
Hepes, Good's Buffer Crystalline
Please see listing in Chemicals for Microscopy.

Hepes Buffer Solution
0.2M
pH range: 7.0-8.0
0-5°C | 11494 | Hepes Buffer Solution | 500 ml | 22.00 | Add to Cart |


HRP Conjugate Diluent
Specifically designed for preparing solution of your HRP-conjugate used as the detection reagent. It is the Antibody-dilutent buffer with additional component for stabilizing your HRP-conjugate. Allows you to further standardize the assay preparing ready-to-use conjugate solutions in advance and store them in refrigerator without loss of activity.
4-8°C | 62716 | HRP Conjugate Diluent | 50 ml | 170.00 | Add to Cart |
4-8°C | 62717 | HRP Conjugate Diluent | 125 ml | 390.00 | Add to Cart |
4-8°C | 62718 | HRP Conjugate Diluent | 500 ml | 1,400.00 | Add to Cart |

Michel's Buffer
RT | 18041-05 | Michel's Buffer | 500 ml | 22.00 | Add to Cart |
RT | 18041-10 | Michel's Buffer | 1 L | 29.00 | Add to Cart |

Millonig's Phosphate Buffer
Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature
The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.
0-5°C | 11582-05 | Millonig's Phosphate Buffer 0.2M | 500 ml | 22.00 | Add to Cart |
0-5°C | 11582-10 | Millonig's Phosphate Buffer 0.2M | 1 L | 31.00 | Add to Cart |

Mops, Biological Buffer
(3-(N-Morpholino) propanesulfonic acid)
pH range 6.5-7.9
0-5°C | 18900 | Mops, Biological Buffer | 100g | 73.00 | Add to Cart |


PHEM Buffer
EMS is proud to announce and release of our newest Buffer for EM PHEM.
PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.
References:
- Schliwa, Manfred, and Jonathan Van Blerkom. "Structural interaction of cytoskeletal components." The Journal of Cell Biology 90.1 (1981): 222-235.
- Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers
2-8°C | 11162 | PHEM Buffer | 225ml | 39.50 | Add to Cart |
2-8°C | 11163 | PHEM Buffer | 500ml | 72.50 | Add to Cart |
Phosphate Buffer, 0.1M*
Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.
19340-60 | Phosphate Buffer, pH 6.0 | ![]() |
1 L | 39.00 | Add to Cart | |
19340-65 | Phosphate Buffer, pH 6.5 | ![]() |
1 L | 39.00 | Add to Cart | |
19340-70 | Phosphate Buffer, pH 7.0 | ![]() |
1 L | 39.00 | Add to Cart | |
19340-72 | Phosphate Buffer, pH 7.2 | ![]() |
1 L | 39.00 | Add to Cart |
*Meant for biological research only. Not to be used for medical or any other purposes.
Phosphate Buffer Saline (PBS)
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
RT | 19342-10 | Phosphate Buffered Saline, 10X | ![]() |
1 L | 30.00 | Add to Cart |
RT | 19242-40 | Phosphate Buffered Saline, 10X | 1 gal | 98.00 | Add to Cart | |
RT | 19343-10 | Phosphate Buffered Saline, 2X | ![]() |
1 L | 27.00 | Add to Cart |
RT | 19343-40 | Phosphate Buffered Saline, 2X | 1 gal | 87.00 | Add to Cart | |
RT | 19344-10 | Phosphate Buffered Saline, 1X | ![]() |
1 L | 23.00 | Add to Cart |
RT | 19344-20 | Phosphate Buffered Saline, 1X | 1 gal | 75.00 | Add to Cart |
Pipes,
Good's Buffer Crystalline
Please see listing in Chemicals for Microscopy.


Pipes Buffer Solution, 0.3M
Aqueous PIPES solution adjusted by 0.1N Sodium Hydroxide to pH 5.5-6.0.
0-5°C | 11610 | Pipes Buffer Solution, 0.3M | 450 ml | 49.50 | Add to Cart |


R-UNIVERSAL Epitope Recovery Buffer (10x stock)
Why the Universal Buffer?
Epitope recovery on formalin-fixed, paraffin embedded tissue sections, requires heat-induced treatment in buffer or, sometimes, proteolitic treatment of the deparaffinized tissue section. Which buffer to use, greatly depends on the exact antibody and the properties of the recognized epitope, therefore, one can find in literature and practice use of many buffers, including Citrate pH 3.4, Citrate 6.0, EDTA 8.0, Tris 9.0- 10.0, Tris-EDTA, etc.
Moreover, for individual antigens also the time of recovery in the individual buffer should be defined, as this may be different, and the treatment often destroys the epitope.
EMS offers a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups.
The buffer was extensively tested in pathology Departments in United Kingdom, and has shown excellent results when used with different antibodies, including those that normally require for successful staining treatment only in Low, or only High pH buffers, or require protolithic treatment.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series).
Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections.
Properties
Clear, non-toxic solution.
Presentation
R-Universal Buffer is supplied as 10x concentrate. For epitope recovery dilute 1 part of stock with 9 parts of deionized water.
Application
For epitope recovery dilute 1 part of stock with 9 parts of deionized water.
Stability and Storage
The preparation is stable for 1 year when stored unopened at +40C. Every lot is issued with a certificate indicating the expiry date.
After opening, store at +40C in the refrigerator and use within 6 months.
Certification
Each lot is certified for compliance to specifications. The product is produced under DIN EN ISO 9001 :2008 Quality Management system for the products in Immunoassay Development and Measurement, Products for Bioanalytics and lmmunoassays.
Ordering Information:
62719-10 | R-UNIVERSAL Epitope Recovery Buffer (10x stock) | 125 ml | 130.00 | Add to Cart |
62719-20 | R-UNIVERSAL Epitope Recovery Buffer (10x stock) | 500 ml | 210.00 | Add to Cart |

Section Block
A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum-based blocking solutions there is no interaction of specific antibody and blocking protein itself. or oth Is not comparable to other commercially available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.
4-8°C | 62710 | Section Block | 50 ml | 150.00 | Add to Cart |
4-8°C | 62711 | Section Block | 125 ml | 265.00 | Add to Cart |
4-8°C | 62712 | Section Block | 500 ml | 1,100.00 | Add to Cart |
Sodium Cacodylate Buffer
Prepared from Sodium Cacodylate Trihydrate (F.W. 214.02)
0-5°C | 11650 | Sodium Cacodylate Buffer, 0.2M, pH 7.4 | ![]() |
225 ml | 29.00 | Add to Cart |
0-5°C | 11652 | Sodium Cacodylate Buffer, 0.2M, pH 7.4 | 500 ml | 42.00 | Add to Cart | |
0-5°C | 11653 | Sodium Cacodylate Buffer, 0.2M, pH 7.4 | 1 L | 84.00 | Add to Cart | |
0-5°C | 11654 | Sodium Cacodylate Buffer, 0.4M, pH 7.2 | ![]() |
500 ml | 60.00 | Add to Cart |
0-5°C | 11655 | Sodium Cacodylate Buffer, 0.4M, pH 7.2 | 1 L | 110.00 | Add to Cart |
Sorensen's Phosphate Buffer
(Phosphate Mixed Sodium Salts)
Sorensen, see Dawson et. al, (1969)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
Sodium Phosphate, monobasic (NaH2PO4xH2O)
0.2M, pH range 5.8 - 8.0
Shipping Conditions: Ambient Temperature
Maximum shelf life after receipt: 3 months
The osmolarity of a 0.1 M at a pH 7.2 is 226 mosmols; with the addition of 0.18 M of sucrose to the solution, the osmolarity raises to 425 mosmols.
Standard pH 7.2; 0.2M is supplied. Otherwise, specify pH when ordering.
0-5°C | 11600-05 | Sorensens Phosphate Buffer, 0.2M, pH 7.2 | ![]() |
500 ml | 22.00 | Add to Cart |
0-5°C | 11600-10 | Sorensens Phosphate Buffer, 0.2M, pH 7.2 | 1 L | 31.00 | Add to Cart | |
0-5°C | 11600-40 | Sorensens Phosphate Buffer, 0.2M, pH 7.2 | 4 L | 108.00 | Add to Cart | |
0-5°C | 11601-10 | Sorensens Phosphate Buffer, 0.2M, pH 7.4 | ![]() |
1 L | 32.00 | Add to Cart |
0-5°C | 11601-40 | Sorensens Phosphate Buffer, 0.2M, pH 7.4 | 4 L | 108.00 | Add to Cart | |
0-5°C | 11682-05 | Sorensen's Phosphate Buffer, 0.15M | ![]() |
500 ml | 25.00 | Add to Cart |
0-5°C | 11682-10 | Sorensen's Phosphate Buffer, 0.15M | 1 L | 32.00 | Add to Cart |


Sym-Collidine Buffer Kit
2,4,6-Trimethylpyridine
N:C(CH3)CH:C(CH3)CH:CCH3 CAS #108-75-8 F.W. 121.18
Bennett & Luft., Biophys & Biochem. Cytol., 6,113 (1959).
A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity. The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200 ml. Preparation Instructions: Pour 9ml of 2N HCl; add 5.34ml of Sym-Collidine, mix well and add distilled water up to 200 ml for a pH 7.4 solution.
Set consists of:
- 1 amp 5.34ml Sym Collidine
- 1 amp 9.0 ml of 2N HCl
0-5°C | 11500 | Sym-Collidine Buffer Kit | Kit | 30.00 | Add to Cart |
0-5°C | 11510 | Sym-Collidine Buffer | 5 kit/bx | 108.00 | Add to Cart |


Sym-Collidine, Reagent
2,4,6-Collidine; 2,4,6-Trimethylpyridine
(C5H2N)(CH3)3 CAS
#108-75-8 F.W. 121.18
Assay: 99% Specific Gravity: 0.9170
RT | 11520 | Sym-Collidine, Reagent | 100 ml | 160.00 | Add to Cart |
Tris, (hydroxymethyl) aminomethane, Crystalline
Please see listing in Chemicals for Microscopy.
Tris, (hydroxymethyl) aminomethane maleate
Tris Maleate Buffer 0.2M pH range 6.4-8.0
0-5°C | 11730 | Tris Maleate Buffer 0.2M pH range 6.4-8.0 | 250 ml | 22.00 | Add to Cart |
0-5°C | 11740 | Tris Maleate Buffer 0.2M pH range 6.4-8.0 | 500 ml | 29.00 | Add to Cart |
Tris Buffer 0.2M pH 8
RT | 11730-05 | Tris Buffer 0.2M pH 8.0 | 250 ml | 22.00 | Add to Cart |
RT | 11730-06 | Tris Buffer 0.2M pH 8.0 | 500 ml | 29.00 | Add to Cart |
Tris Buffered Saline pH 7.4
RT | 11730-08 | Tris Buffered Saline pH 7.4 | 250 ml | 22.00 | Add to Cart |
RT | 11730-09 | Tris Buffered Saline pH 7.4 | 500 ml | 29.00 | Add to Cart |
Tris-HCL Buffer1M pH 6-8
RT | 11730-12 | Tris-HCL Buffer 1M pH range 6-8 | 250 ml | 22.00 | Add to Cart |
RT | 11730-15 | Tris-HCL Buffer 1M pH range 6-8 | 500 ml | 29.00 | Add to Cart |
Tris HCL Buffer pH range 7.2 - 9.0
RT | 11730-20 | Tris HCL Buffer pH range 7.2-9.0 | 250 ml | 22.00 | Add to Cart |
RT | 11730-25 | Tris HCL Buffer pH range 7.2-9.0 | 500 ml | 29.00 | Add to Cart |

Tris Buffer pH 10.0 (10x)
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C
4-8°C | 64142-11 | Tris Buffer pH 10.0 | 100 ml | 75.00 | Add to Cart |
4-8°C | 64142-12 | Tris Buffer pH 10.0 | 1000 ml | 125.00 | Add to Cart |

Universal Immunoassay Buffer™, 10x
Immunoassay buffer is used as a 1x in all immunoassays (immunofluorescence, IHC, ELISA, WB etc.) as a washing buffer and it can be used as a dilution buffer for antibodies; however it does not contain any carrier proteins.
This buffer also does not contain any phosphate, sodium azide or mercury as preservatives and can be used for dilution of all antibodies, including peroxidase, antibodies to phosphoproteins. It is not suitable for dilution of antibodies to S100 proteins.
Reagent Supplied: 10x Buffer
Application: Dilute this 10x buffer 10 times with DI water. (90 ml water + 10 ml this buffer – mixed well). Wash 3-5 times with this buffer in between the steps of your immunoassay protocol.
Storage: Room temperature
RT | 25880-01 | Universal Immunoassay Buffer, 10x | 100 ml | 56.00 | Add to Cart |
RT | 25880-10 | Universal Immunoassay Buffer, 10x | 1 L | 158.00 | Add to Cart |

Universal Antibody Dilution Buffer™
This ready-to-use Universal Antibody Dilution Buffer is designed for dilution of antibodies in all immunoassays, including immunofluorescence, IHC, ELISA, WB. This buffer does not contain any mammalian proteins, phosphate, sodium azide, or mercury preservatives and can be used for dilution of all antibodies, including peroxidase, and antibodies to phophoproteins. However, it is not suitable for dilution of antibodies to S100 proteins.
Reagent Supplied: Ready to use buffer
Application: Dilute antibodies, primary or secondary as recommended in the protocol by the manufacturer of these products.
Storage: 2–8°C. Diluted antibodies can be stored at 2–8°C for several weeks, depending upon the stability of antibodies.
2-8°C | 25885-01 | Universal Antibody Dilution Buffer | 100 ml | 56.00 | Add to Cart |
2-8°C | 25885-05 | Universal Antibody Dilution Buffer | 500 ml | 160.00 | Add to Cart |