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arrow13Glutaraldehyde - The Highest Purity

Glutaraldehydearrow12 Glutaraldehyde

OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

  • The Universal Fixative for Electron Microscopy.
  • Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
  • THE HIGHEST PURITY, free from polymers and other contaminants.
  • Each lot is tested and assayed in our lab before we ship and guarantee its purity.
  • Sealed under dry nitrogen.
  • Refrigerated prior to shipment.

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Micro-scopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235nm rather than the 280nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

msds8% Aqueous Solution
0-5°C 16000   10 ml ampoule 6.00 Add to Cart
0-5°C 16019   Box 10x2ml 22.00 Add to Cart
0-5°C 16020   Box 10 x 10 ml ampoules 26.00 Add to Cart
0-5°C 16021 same as 16020 10 bxs 250.00 Add to Cart
0-5°C 16022 same as 16020 20 bxs 425.00 Add to Cart
0-5°C 16010   100 ml bottle 24.00 Add to Cart
msds10% Aqueous Solution
0-5°C 16100   10 ml ampoule 6.00 Add to Cart
0-5°C 16120   Box 10 x 10 ml ampoules 28.00 Add to Cart
0-5°C 16121 same as 16120 10 bxs 266.00 Add to Cart
0-5°C 16122 same as 16120 20 bxs 452.00 Add to Cart
0-5°C 16110   100 ml bottle 24.00 Add to Cart
msds25% Aqueous Solution
0-5°C 16200   10 ml ampoule 7.00 Add to Cart
0-5°C 16220   Box 10 x 10 ml ampoules 31.00 Add to Cart
0-5°C 16221 same as 16220 10 bxs 305.00 Add to Cart
0-5°C 16222 same as 16220 20 bxs 530.00 Add to Cart
0-5°C 16210   100 ml bottle 24.00 Add to Cart
msds50% Aqueous Solution
0-5°C 16300   10 ml ampoule 7.50 Add to Cart
0-5°C 16320   Box 10 x 10 ml ampoules 44.00 Add to Cart
0-5°C 16321 same as 16320 10 bxs 420.00 Add to Cart
0-5°C 16322 same as 16320 20 bxs 760.00 Add to Cart
0-5°C 16310   100 ml bottle 42.00 Add to Cart
msds70% Aqueous Solution
0-5°C 16350   10 ml ampoule 8.50 Add to Cart
0-5°C 16360   box 10 x 10 ml ampoules 59.00 Add to Cart
0-5°C 16365   box 10 x 2ml ampoules 34.00 Add to Cart
msdsmsdsBiological Grade
0-5°C 16400 25% Solution 450 ml 19.00 Add to Cart
0-5°C 16401 25% Solution 4x450 ml 72.00 Add to Cart
0-5°C 16410 25% Solution 1 gal. 73.00 Add to Cart
0-5°C 16411 25% Solution 4x1 gal. 264.00 Add to Cart
0-5°C 16500 50% Solution 450 ml 22.00 Add to Cart
0-5°C 16501 50% Solution 4x450 ml 78.00 Add to Cart
0-5°C 16520 50% Solution 1 liter 42.00 Add to Cart
0-5°C 16521 50% Solution 4x1 liter 150.00 Add to Cart

msdsmsdsarrow12Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

0-5°C 16216 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 44.00 Add to Cart
0-5°C 16214 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 36.00 Add to Cart
0-5°C 16316 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 55.00 Add to Cart
0-5°C 16314 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 45.00 Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

arrow12Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792

Ordering Information: (please call for additional information).

0-5°C 16530 10% in Acetone msds 10x10 ml 86.00 Add to Cart
0-5°C 16531 10% in Ethanol msds 10x10 ml 86.00 Add to Cart
0-5°C 16532 10% in Methanol msds 10x10 ml 86.00 Add to Cart
0-5°C 16533 10% in Solvent of your choice   10x10 ml 86.00 Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

arrow12Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

msdsGlutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16535-05 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 500 ml 140.00 Add to Cart
0-5°C 16535-10 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 1 L 268.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16536-05 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 142.00 Add to Cart
0-5°C 16536-10 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 1 L 277.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16536-15 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 500 ml 152.00 Add to Cart
0-5°C 16536-20 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 1 l 288.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16537-05 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 500 ml 158.00 Add to Cart
0-5°C 16537-10 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 1 l 292.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16537-15 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 500 ml 158.00 Add to Cart
0-5°C 16537-20 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 1 L 298.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16538-05 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 164.00 Add to Cart
0-5°C 16538-10 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 1 l 298.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4
0-5°C 16538-15 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 500 ml 166.00 Add to Cart
0-5°C 16538-20 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 1 l 302.00 Add to Cart
msdsGlutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2
0-5°C 16539-45 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 10x10 ml 37.00 Add to Cart
0-5°C 16539-50 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 500 ml 136.00 Add to Cart
0-5°C 16539-55 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 1 L 278.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3
0-5°C 16539-60 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 10x10 ml 38.00 Add to Cart
0-5°C 16539-65 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 500 ml 136.00 Add to Cart
0-5°C 16539-70 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 1 L 275.00 Add to Cart
msdsGlutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4
0-5°C 16536-06 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 100 ml 38.00 Add to Cart
0-5°C 16536-07 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 157.00 Add to Cart
0-5°C 16536-08 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 1 Liter 283.00 Add to Cart
msdsGlutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4
0-5°C 16537-06 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 100 ml 36.00 Add to Cart
0-5°C 16537-07 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 500 ml 155.00 Add to Cart
0-5°C 16537-08 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 1 Liter 280.00 Add to Cart
msdsGlutaraldehyde 2.5% Aqueous
0-5°C 16537-16 Glutaraldehyde 2.5%, Aqueous 10x10 ml 24.00 Add to Cart
0-5°C 16537-17 Glutaraldehyde 2.5% Aqueous 500 ml 66.00 Add to Cart
0-5°C 16537-18 Glutaraldehyde 2.5% Aqueous 1 Liter 116.00 Add to Cart
msdsGlutaraldehyde 3.0% Aqueous
0-5°C 16537-19 Glutaraldehyde 3% Aqueous 100 ml 26.00 Add to Cart
0-5°C 16537-20-S Glutaraldehyde 3% Aqueous 500 ml 66.00 Add to Cart
0-5°C 16537-21 Glutaraldehyde 3% Aqueous 1 Liter 116.00 Add to Cart
msdsGlutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16538-06 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 10x10 ml 26.00 Add to Cart
0-5°C 16538-07 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 66.00 Add to Cart
0-5°C 16538-08 Glutaraldehyde 3% Paraformaldehyde 3% in  Phosphate Buffer, pH 7.4 1 Liter 96.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4
0-5°C 16539-06 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 37.00 Add to Cart
0-5°C 16539-07 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 500 ml 137.00 Add to Cart
0-5°C 16539-08 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 1 Liter 278.00 Add to Cart
msdsGlutaraldehyde 5% Aqueous
0-5°C 16539-15 Glutaraldehyde 5% Aqueous 10x10 ml 25.00 Add to Cart
0-5°C 16539-20 Glutaraldehyde 5% Aqueous 500 ml 66.00 Add to Cart
0-5°C 16539-25 Glutaraldehyde 5% Aqueous 1 Liter 116.00 Add to Cart
msdsGlutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4
0-5°C 16539-30 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 10x10 ml 38.00 Add to Cart
0-5°C 16539-35 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 500 ml 136.00 Add to Cart
0-5°C 16539-40 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 1 Liter 276.00 Add to Cart

arrow12Glutaraldehyde/Formaldehyde

msdsFixative (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

RT 15731-10 Karnovsky Fixative Reagent Grade 1 L 256.00 Add to Cart
msdsKarnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT 15732-10 Karnovsky Fixative EM Grade 1 L 282.00 Add to Cart

Ampoule Crackerarrow12Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.

60605-04 Ampoule Cracker for Ampoule Size 1 – 4 ml 500/pk 39.00 Add to Cart
60605-10 Ampoule Cracker for Ampoule Size 5 – 10 ml 500/pk 39.00 Add to Cart
60605-20 Ampoule Cracker for Ampoule Size 10 – 20 ml 500/pk 74.00 Add to Cart

Break-Safe Ampoule OpenerBreak-Safe Ampoule opener

arrow12Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.

Box style design allows one handed opening of up to 3 ampules at a time. Gently pressing down on the lid snaps ampule necks clean while keeping them contained within the device and away from fingers. After removing ampule contents, simply turn the device upside down over a trash receptacle to safely discard ampules and tops.

Features:

  • Includes 4 inserts to allow opening of 1ml, 2ml, 5ml and 10 ml ampules.
  • Open ampules easily and safely.
  • Transparency allows unobstructed viewing of ampule contents at all times.
  • Copolyester plastic construction has broad chemical resistance.
60607 Break-Safe™ Ampoule Opener each 46.50 Add to Cart

arrow12Ampoule Breaker; Soft Silicon Ampoule Breaker; Soft Silicone

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600 Ampoule Breaker each 16.00 Add to Cart

msdsarrow12HISTOCHOICE® Clearing Agent

Histology Grade - Non flammable, replacement for Xylene for all tissue clearing needs.

64114-01 Histochoice Clearing Agent 4 L 62.00 Add to Cart
64114-04 Histochoice Clearing Agent 4x4 L 240.00 Add to Cart

msdsarrow12HISTOCHOICE™MB®* – The Molecular Biology Fixative

  • SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury.
  • NON-TOXIC – Is completely odorless and can be safely disposed of down the drain.

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

RT 64115-01 HISTOCHOICE™MB® Fixative 1 L 60.00 Add to Cart
RT 64115-04 HISTOCHOICE™MB® Fixative 4 L 190.00 Add to Cart
RT 64115-44 HISTOCHOICE™MB® Fixative 4x4 L 695.00 Add to Cart

* HISTOCHOICE is registered name of Amresco

msdsarrow12Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

  Catalog # Cup Size Filled Pack/cs Price  
RT 64120-10 15 ml 10 ml 50 78.00 Add to Cart
RT 64120-20 30 ml 20 ml 50 98.00 Add to Cart
RT 64120-30 60 ml 30 ml 20 46.00 Add to Cart

Bulk packaging is available:

RT 64120-01 Hollande's Fixative 1 L 44.00 Add to Cart
RT 64120-04 Hollande's Fixative 4 L 160.00 Add to Cart

msdsarrow12McDowell Trumps Fixative

RT 18030-05 McDowell Trumps Fixative 500 ml 32.00 Add to Cart
RT 18030-10 McDowell Trumps Fixative 1 L 38.00 Add to Cart

msdsarrow12Michel's Fixative, pH 7.0 – 7.4

RT 18040-05 Michel's Fixative 500 ml 49.00 Add to Cart
RT 18040-10 Michel's Fixative 1 L 84.00 Add to Cart
RT 18040-20 Michel's Fixative Pre-filled 20 x10 ml 74.00 Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

15560arrow12Mirsky's Fixative™

  • Saponin/Glyoxylate Fixative
  • Preserves Immunohistological activity
  • Contains no formaldehyde
  • Fast-acting

Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem “raw”. This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.

Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shelf life of 30 days.

RT 15560 Mirsky's Fixative System msds
msds		 200 ml 36.00 Add to Cart
RT 15561-05 Mirsky's Fixative Ready-to-Use in Ethanol msds 500 ml 17.00 Add to Cart
RT 15561-10 Mirsky's Fixative Ready-to-Use in Methanol 1L 32.00 Add to Cart
RT 15561-20 Mirsky's Fixative Ready-to-Use in Solvent of your choice 2L 60.00 Add to Cart

msdsarrow12Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

RT 64123-05 Russell's Zenker Fixative 500 ml 26.00 Add to Cart
RT 64123-06 Russell's Zenker Fixative 1 L 43.00 Add to Cart

Technical Tips

The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer

Certificates of Analysis


Glycerine to HPMA arrow13arrow13

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© 2018 Electron Microscopy Sciences

Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology.