Certificates of Analysis

Glutaraldehyde – The Highest Purity

Glutaraldehyde

OCHCH₂CH₂CH₂CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

• The Universal Fixative for Electron Microscopy
• Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
• THE HIGHEST PURITY, free from polymers and other contaminants
• Each lot is tested and assayed in our lab before we ship and guarantee its purity
• Sealed under dry nitrogen
• Refrigerated prior to shipment

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235 nm rather than the 280 nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

8% Aqueous Solution

10% Aqueous Solution

25% Aqueous Solution

50% Aqueous Solution

70% Aqueous Solution

Biological Grade

Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792

Ordering Information: (please call for additional information).

0-5°C	16530	10% in Acetone		10x10 ml	88.00	Add to Cart
0-5°C	16531	10% in Ethanol		10x10 ml	88.00	Add to Cart
0-5°C	16532	10% in Methanol		10x10 ml	88.00	Add to Cart
0-5°C	16533	10% in Solvent of your choice		10x10 ml	88.00	Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

Glutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4

Glutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4

Glutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4

Glutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2

Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3

Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4

Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4

Glutaraldehyde 2.5% Aqueous

Glutaraldehyde 3.0% Aqueous

Glutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4

Glutaraldehyde 5% Aqueous

Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4

Glutaraldehyde/Formaldehyde

Fixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

Karnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

Ampoule Breaker; Soft Silicone

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.

Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.

Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoule necks clean while keeping them contained within the device and away from fingers. After removing ampoule contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.

Features

• Includes 4 inserts to allow opening of 1 ml, 2 ml, 5 ml and 10 ml ampoules
• Open ampoules easily and safely
• Transparency allows unobstructed viewing of ampoule contents at all times
• Copolyester plastic construction has broad chemical resistance

HISTOCHOICE® Clearing Agent

Histology Grade – Non flammable, replacement for Xylene for all tissue clearing needs.

HISTOCHOICE® Clearing Agent is on long-term backorder... please check back for updates or select catalog numbers 64110 or 64111 as a substitute.

HISTOCHOICE™MB®* – The Molecular Biology Fixative

• SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury
• NON-TOXIC – Is completely odorless and can be safely disposed of down the drain

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

HISTOCHOICE™MB® is on long-term backorder... please check back for updates.

* HISTOCHOICE is registered name of Amresco

Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

Bulk packaging available

McDowell Trumps Fixative

Michel's Fixative, pH 7.0 – 7.4

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

Michel's Transport Medium

Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature. Can be used with Michel's Buffer (washing solution).

Mirsky's Fixative™

• Saponin/Glyoxylate Fixative
• Preserves Immunohistological activity
• Contains no formaldehyde
• Fast-acting

Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5 cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.

Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shelf life of 30 days.

RT	15560	Mirsky's Fixative System		200 ml	42.00	Add to Cart
RT	15561-05	Mirsky's Fixative Ready-to-Use in Ethanol		500 ml	24.00	Add to Cart
RT	15561-10	Mirsky's Fixative Ready-to-Use in Methanol		1L	42.00	Add to Cart
RT	15561-20	Mirsky's Fixative Ready-to-Use in Solvent of your choice		2L	82.00	Add to Cart

Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In its place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

Technical Tips

The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer

Certificates of Analysis

Glycerine to HPMA