OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131
EM Grade Distillation Purified
The Universal Fixative for Electron Microscopy
Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
THE HIGHEST PURITY, free from polymers and other contaminants
Each lot is tested and assayed in our lab before we ship and guarantee its purity
Sealed under dry nitrogen
Refrigerated prior to shipment
After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.
Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.
We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.
EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235 nm rather than the 280 nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.
Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.
* All other concentrations are available in serum vials upon request.
For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.
Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution
Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792
Ordering Information: (please call for additional information).
Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.
The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.
EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.
Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoule necks clean while keeping them contained within the device and away from fingers. After removing ampoule contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.
Features
Includes 4 inserts to allow opening of 1 ml, 2 ml, 5 ml and 10 ml ampoules
Open ampoules easily and safely
Transparency allows unobstructed viewing of ampoule contents at all times
Copolyester plastic construction has broad chemical resistance
A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.
Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.
Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature. Can be used with Michel's Buffer (washing solution).
Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.
Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5 cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.
Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.
The two bottle system has a shelf life of 12 months while the ready-to-use format has a shelf life of 30 days.
Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In its place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.