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Electron Microscopy Sciences

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arrow13Glutaraldehyde – The Highest Purity

Glutaraldehydearrow12Glutaraldehyde

OCHCH2CH2CH2CHO F.W. 100.12 CAS #111-30-8
Specific Gravity @20/20°C: 1.131

EM Grade Distillation Purified

  • The Universal Fixative for Electron Microscopy
  • Purified to EM Grade from commercial grade by "all-glass vacuum-distillation."
  • THE HIGHEST PURITY, free from polymers and other contaminants
  • Each lot is tested and assayed in our lab before we ship and guarantee its purity
  • Sealed under dry nitrogen
  • Refrigerated prior to shipment

After glutaraldehyde has been purified to EM grade, it has a high risk of polymerizing if it is not handled properly. Electron Microscopy Sciences takes special precautions to assure a polymer-free, pure glutaraldehyde.

Every lot of our EM grade aqueous glutaraldehyde is assayed prior to shipping assuring you consistent purity and the highest possible quality. If stored properly, our Glutaraldehyde has a very long shelf life.

We package our aqueous glutaraldehyde in 10 ml, single dose ampoules, sealed under dry nitrogen, and kept refrigerated until it is shipped. We offer our glutaraldehyde in a wide range of percentages to simplify mixing procedures and avoiding waste.

EM grade aqueous glutaraldehyde, stored in volumes greater than 100 ml, with repeated defrosting and recapping, will polymerize in time, producing an absorption peak of 235 nm rather than the 280 nm of pure EM grade. The presence of polymerized glutaraldehyde greatly reduces its efficiency as a cross-linking agent, preventing proper fixation. For this reason, we provide 10 ml ampoules and 100 ml bottle quantities for larger applications.

msds8% Aqueous Solution
4 to (-18)°C 16000   10 ml ampoule 6.50 Add to Cart
4 to (-18)°C 16019   Box 10x2ml 26.00 Add to Cart
4 to (-18)°C 16020   Box 10 x 10 ml ampoules 30.00 Add to Cart
4 to (-18)°C 16021 same as 16020 10 bxs 290.00 Add to Cart
4 to (-18)°C 16022 same as 16020 20 bxs 485.00 Add to Cart
4 to (-18)°C 16010   100 ml bottle 28.00 Add to Cart
4 to (-18)°C 16010-1L   1 L 232.00 Add to Cart
msds10% Aqueous Solution
4 to (-18)°C 16100   10 ml ampoule 6.50 Add to Cart
4 to (-18)°C 16120   Box 10 x 10 ml ampoules 33.00 Add to Cart
4 to (-18)°C 16121 same as 16120 10 bxs 310.00 Add to Cart
4 to (-18)°C 16122 same as 16120 20 bxs 535.00 Add to Cart
4 to (-18)°C 16110   100 ml bottle 28.00 Add to Cart
4 to (-18)°C 16110-1L   1 L 232.00 Add to Cart
msds25% Aqueous Solution
4 to (-18)°C 16200   10 ml ampoule 8.00 Add to Cart
4 to (-18)°C 16220   Box 10 x 10 ml ampoules 36.00 Add to Cart
4 to (-18)°C 16221 same as 16220 10 bxs 340.00 Add to Cart
4 to (-18)°C 16222 same as 16220 20 bxs 588.00 Add to Cart
4 to (-18)°C 16210   100 ml bottle 28.00 Add to Cart
4 to (-18)°C 16210-1L   1 L 250.00 Add to Cart
msds50% Aqueous Solution
4 to (-18)°C 16300   10 ml ampoule 8.00 Add to Cart
4 to (-18)°C 16320   Box 10 x 10 ml ampoules 48.00 Add to Cart
4 to (-18)°C 16321 same as 16320 10 bxs 460.00 Add to Cart
4 to (-18)°C 16322 same as 16320 20 bxs 830.00 Add to Cart
4 to (-18)°C 16310   100 ml bottle 46.00 Add to Cart
4 to (-18)°C 16310-1L   1 L 365.00 Add to Cart
msds70% Aqueous Solution
4 to (-18)°C 16350   10 ml ampoule 9.00 Add to Cart
4 to (-18)°C 16360   box 10 x 10 ml ampoules 66.00 Add to Cart
4 to (-18)°C 16365   box 10 x 2ml ampoules 40.00 Add to Cart
msdsmsdsBiological Grade
4 to (-18)°C 16400 25% Solution 450 ml 23.00 Add to Cart
4 to (-18)°C 16401 25% Solution 4x450 ml 88.00 Add to Cart
4 to (-18)°C 16410 25% Solution 1 gal. 90.00 Add to Cart
4 to (-18)°C 16411 25% Solution 4x1 gal. 342.00 Add to Cart
4 to (-18)°C 16500 50%Solution 450 ml 26.00 Add to Cart
4 to (-18)°C 16501 50% Solution 4x450 ml 99.50 Add to Cart
4 to (-18)°C 16520 50% Solution 1 liter 49.50 Add to Cart
4 to (-18)°C 16521 50% Solution 4x1 liter 186.00 Add to Cart

msdsmsdsarrow12Serum Vial Aqueous Glutaraldehyde

Over the years, many of our valued customers have requested purchasing our aqueous Glutaraldehyde in serum vials. This packaging allows the user to puncture the special non-reactive seal with a hypodermic syringe and draw out the exact quantity of aqueous Glutaraldehyde needed without opening the container. This packaging assures freshness and purity of the aqueous Glutaraldehyde at all times.

4 to (-18)°C 16216 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 49.00 Add to Cart
4 to (-18)°C 16214 25% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 42.00 Add to Cart
4 to (-18)°C 16316 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 10 x10 ml 62.00 Add to Cart
4 to (-18)°C 16314 50% EM Grade Aqueous Glutaraldehyde in Serum Vial 100 ml 50.00 Add to Cart

* All other concentrations are available in serum vials upon request.

For any special concentration, or packaging requirements other than those already listed, please call us today. We will manufacture in accordance to your specifications.

arrow12Anhydrous EM Grade Glutaraldehyde: Specially Prepared-10% Solution

Rapid-Freezing on a polished metal surface, at the temp. of liquid helium or liquid nitrogen, followed by freeze-substitution in osmium-acetone, has been shown to be a very useful technique for capturing rapid cellular events and for preserving cell ultrastructure.
* John M. Robinson and Morris J. Karnovsky, 1991. "Rapid-freezing Cytochemistry: Preservation of Tubular Lysosomes and Enzyme Activity". J. of Histochem., Cytochem. Vol. 39, No. 6, pp 787-792

Ordering Information: (please call for additional information).

0-5°C 16530 10% in Acetone msds 10x10 ml 90.00 Add to Cart
0-5°C 16531 10% in Ethanol msds 10x10 ml 90.00 Add to Cart
0-5°C 16532 10% in Methanol msds 10x10 ml 90.00 Add to Cart
0-5°C 16533 10% in Solvent of your choice   10x10 ml 90.00 Add to Cart

We would be pleased to fill your specific glutaraldehyde needs. Please call for prices on special concentrations or size requirements.

arrow12Glutaraldehyde, Pre-Made, Ready-to-Use, in Buffer

msdsGlutaraldehyde 0.625% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16535-05 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 500 ml 145.00 Add to Cart
2-8°C 16535-10 Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 1 L 275.00 Add to Cart
2-8°C 16535-10-1G Glutaraldehyde 0.625% in Phosphate Buffer, pH 7.4 1 Gal. 875.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16536-05 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 146.00 Add to Cart
2-8°C 16536-10 Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 1 L 282.00 Add to Cart
2-8°C 16536-10-1G Glutaraldehyde 2% in Phosphate Buffer, pH 7.4 1 Gal. 880.00 Add to Cart
msdsGlutaraldehyde 2% in 0.1M Sodium Cacodylate Buffer, pH 7.4
2-8°C 16536-15 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 500 ml 156.00 Add to Cart
2-8°C 16536-20 Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 1 L 294.00 Add to Cart
2-8°C 16536-20-1G Glutaraldehyde 2% in Sodium Cacodylate Buffer, pH 7.4 1 Gal. 925.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16537-05 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 500 ml 164.00 Add to Cart
2-8°C 16537-10 Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 1 L 296.00 Add to Cart
2-8°C 16537-10-1G Glutaraldehyde 2.5% in Phosphate Buffer, pH 7.4 1 Gal. 940.00 Add to Cart
msdsGlutaraldehyde 2.5% in 0.1M Sodium Cacodylate Buffer, pH 7.4
2-8°C 16537-15 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 500 ml 162.00 Add to Cart
2-8°C 16537-20 Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 1 L 302.00 Add to Cart
2-8°C 16537-20-1G Glutaraldehyde 2.5% in Sodium Cacodylate Buffer, pH 7.4 1 Gal. 1000.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16538-05 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 168.00 Add to Cart
2-8°C 16538-10 Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 1 L 302.00 Add to Cart
2-8°C 16538-10-1G Glutaraldehyde 3% in Phosphate Buffer, pH 7.4 1 Gal. 1000.00 Add to Cart
msdsGlutaraldehyde 3% in 0.1M Sodium Cacodylate Buffer, pH 7.4
2-8°C 16538-15 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 500 ml 170.00 Add to Cart
2-8°C 16538-20 Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 1 L 307.00 Add to Cart
2-8°C 16535-20-1G Glutaraldehyde 3% in Sodium Cacodylate Buffer, pH 7.4 1 Gal. 1010.00 Add to Cart
msdsGlutaraldehyde 3% Sodium Phosphate Buffer, pH 7.2
2-8°C 16539-45 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 10x10 ml 41.00 Add to Cart
2-8°C 16539-50 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 500 ml 140.00 Add to Cart
2-8°C 16539-55 Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 1 L 284.00 Add to Cart
2-8°C 16539-55-1G Glutaraldehyde 3% in Sodium Phosphate Buffer, pH 7.2 1 Gal. 975.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3
2-8°C 16539-60 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 10x10 ml 43.00 Add to Cart
2-8°C 16539-65 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 500 ml 140.00 Add to Cart
2-8°C 16539-70 Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 1 L 280.00 Add to Cart
2-8°C 16539-70-1G Glutaraldehyde 4% in 0.1m Phosphate Buffer, pH 7.3 1 Gal. 850.00 Add to Cart
msdsGlutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4
2-8°C 16536-06 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 100 ml 42.00 Add to Cart
2-8°C 16536-07 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 500 ml 161.00 Add to Cart
2-8°C 16536-08 Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 1 L 288.00 Add to Cart
2-8°C 16536-08-1G Glutaraldehyde 2% Paraformaldehyde 2% in Phosphate Buffer, pH 7.4 1 Gal. 915.00 Add to Cart
msdsGlutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4
2-8°C 16537-06 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 100 ml 40.00 Add to Cart
2-8°C 16537-07 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 500 ml 160.00 Add to Cart
2-8°C 16537-08 Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 1 L 286.00 Add to Cart
2-8°C 16537-08-1G Glutaraldehyde 2.5% Paraformaldehyde 1% in Phosphate Buffer, pH 7.4 1 Gal. 914.00 Add to Cart
msdsGlutaraldehyde 2.5% Aqueous
2-8°C 16537-16 Glutaraldehyde 2.5% Aqueous 10x10 ml 27.00 Add to Cart
2-8°C 16537-17 Glutaraldehyde 2.5% Aqueous 500 ml 70.00 Add to Cart
2-8°C 16537-18 Glutaraldehyde 2.5% Aqueous 1 L 120.00 Add to Cart
2-8°C 16537-18-1G Glutaraldehyde 2.5% Aqueous 1 Gal. 395.00 Add to Cart
msdsGlutaraldehyde 3.0% Aqueous
2-8°C 16537-19 Glutaraldehyde 3% Aqueous 100 ml 30.00 Add to Cart
2-8°C 16537-20-S Glutaraldehyde 3% Aqueous 500 ml 70.00 Add to Cart
2-8°C 16537-21 Glutaraldehyde 3% Aqueous 1 L 120.00 Add to Cart
2-8°C 16537-21-1G Glutaraldehyde 3% Aqueous 1 Gal. 395.00 Add to Cart
msdsGlutaraldehyde 3.0% Paraformaldehyde 3% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16538-06 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 10x10 ml 30.00 Add to Cart
2-8°C 16538-07 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 500 ml 70.00 Add to Cart
2-8°C 16538-08 Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 1 L 100.00 Add to Cart
2-8°C 16538-08-1G Glutaraldehyde 3% Paraformaldehyde 3% in Phosphate Buffer, pH 7.4 1 Gal. 350.00 Add to Cart
msdsGlutaraldehyde 4% in 0.1M in Sodium Cacodylate Buffer, pH 7.4
2-8°C 16539-06 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 42.00 Add to Cart
2-8°C 16539-07 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 500 ml 142.00 Add to Cart
2-8°C 16539-08 Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 1 L 282.00 Add to Cart
2-8°C 16539-08-1G Glutaraldehyde 4% in 0.1M Sodium Cacodylate Buffer, pH 7.4 1 Gal. 912.00 Add to Cart
msdsGlutaraldehyde 5% Aqueous
2-8°C 16539-15 Glutaraldehyde 5% Aqueous 10x10 ml 29.00 Add to Cart
2-8°C 16539-20 Glutaraldehyde 5% Aqueous 500 ml 70.00 Add to Cart
2-8°C 16539-25 Glutaraldehyde 5% Aqueous 1 L 120.00 Add to Cart
2-8°C 16539-25-1G Glutaraldehyde 5% Aqueous 1 Gal. 395.00 Add to Cart
msdsGlutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4
2-8°C 16539-30 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 10x10 ml 42.00 Add to Cart
2-8°C 16539-35 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 500 ml 140.00 Add to Cart
2-8°C 16539-40 Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 1 L 280.00 Add to Cart
2-8°C 16539-40-1G Glutaraldehyde 5% in 0.1M Phosphate Buffer, pH 7.4 1 Gal. 910.00 Add to Cart

arrow12Glutaraldehyde/Formaldehyde

msdsFixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

2-8°C 15731-10 Karnovsky's Fixative Reagent Grade 1 L 268.00 Add to Cart
2-8°C 15731-10-1G Karnovsky's Fixative Reagent Grade 1 Gal. 825.00 Add to Cart
2-8°C 15731-10-5G Karnovsky's Fixative Reagent Grade 5 Gal. 2000.00 Add to Cart
msdsKarnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

2-8°C 15732-10 Karnovsky's Fixative EM Grade 1 L 290.00 Add to Cart
2-8°C 15732-10-1G Karnovsky's Fixative EM Grade 1 Gal. 850.00 Add to Cart
2-8°C 15732-10-5G Karnovsky's Fixative EM Grade 5 Gal. 2200.00 Add to Cart
Ampoule Breaker; Soft Silicon

arrow12Ampoule Breaker; Soft Silicone

Reusable – Made from soft silicone. It offers a simple way to break pre-scored glass ampoules safely. Accommodates 5, 7, and 10 ml size ampoules. Reduces injury and spillage.

60600 Ampoule Breaker each 17.00 Add to Cart

Ampoule Crackerarrow12Ampoule Cracker™

The Ampoule Cracker™ is a disposable plastic fracture and safety collar, which is specially designed to aid in the breaking open of pre-scored glass ampoules. Simply slip the Ampoule Cracker™ over the top at the pre-scored portion, snap it, and discard both the ampoule top and the safety collar to prevent cross contamination.

60605-04 Ampoule Cracker for Ampoule Size 1 – 4 ml 500/pk 44.00 Add to Cart
60605-10 Ampoule Cracker for Ampoule Size 5 – 10 ml 500/pk 44.00 Add to Cart
60605-20 Ampoule Cracker for Ampoule Size 10 – 20 ml 500/pk 80.00 Add to Cart

Break-Safe Ampoule OpenerBreak-Safe Ampoule opener

arrow12Break-Safe™ Ampoule Opener

EMS Introduces Break-Safe™ allowing you to safely open up to 3 ampoules at one time.

Box style design allows one handed opening of up to 3 ampoules at a time. Gently pressing down on the lid snaps ampoule necks clean while keeping them contained within the device and away from fingers. After removing ampoule contents, simply turn the device upside down over a trash receptacle to safely discard ampoules and tops.

Features

  • Includes 4 inserts to allow opening of 1 ml, 2 ml, 5 ml and 10 ml ampoules
  • Open ampoules easily and safely
  • Transparency allows unobstructed viewing of ampoule contents at all times
  • Copolyester plastic construction has broad chemical resistance
60607 Break-Safe™ Ampoule Opener each 52.00 Add to Cart

msdsarrow12Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

  Catalog # Cup Size Filled Pack/cs Price  
RT 64120-10 15 ml 10 ml 50 78.00 Add to Cart
RT 64120-20 30 ml 20 ml 50 98.00 Add to Cart
RT 64120-30 60 ml 30 ml 20 46.00 Add to Cart
Bulk packaging available
RT 64120-01 Hollande's Fixative 1 L 46.00 Add to Cart
RT 64120-04 Hollande's Fixative 4 L 168.00 Add to Cart
RT 64120-01-5G Hollande's Fixative 5 Gal. 675.00 Add to Cart

msdsarrow11McDowell Trumps Fixative

RT 18030-05 McDowell Trumps Fixative 500 ml 33.00 Add to Cart
RT 18030-10 McDowell Trumps Fixative 1 L 39.00 Add to Cart
RT 18030-10-1G McDowell Trumps Fixative 1 Gal. 160.00 Add to Cart
RT 18030-10-5G McDowell Trumps Fixative 5 Gal. 600.00 Add to Cart

msdsarrow12Michel's Fixative, pH 7.0 – 7.4

2-8°C 18040-05 Michel's Fixative 500 ml 52.00 Add to Cart
2-8°C 18040-10 Michel's Fixative 1 L 86.00 Add to Cart
2-8°C 18040-20 Michel's Fixative Pre-filled 20 x 10 ml 76.00 Add to Cart
2-8°C 18040-10-1G Michel's Fixative 1 Gal. 250.00 Add to Cart
2-8°C 18040-10-5G Michel's Fixative 5 Gal. 624.00 Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

arrow12Michel's Transport Medium

Michel's Transport Medium allows for the shipping of specimens for further immunofluorescence work. It preserves morphology and antigenicity during transport. It is not a buffer or fixative. It is simply a vehicle to transport samples. The solution must be stored at refrigerated temperatures. Do not freeze. Samples may be stored up to 5 days in this medium at room temperature. Can be used with Michel's Buffer (washing solution).

2-8°C 18042-05 Michel's Transport Medium 250 ml 29.00 Add to Cart
2-8°C 18042-10 Michel's Transport Medium 500 ml 49.00 Add to Cart
2-8°C 18042-15 Michel's Transport Medium 1 L 66.00 Add to Cart
2-8°C 18042-16 Michel's Transport Medium 1 gal. 144.00 Add to Cart
2-8°C 18042-17 Michel's Transport Medium, 15ml fill in SecurTainer™ 50/cs 82.00 Add to Cart
2-8°C 18042-16-5G Michel's Transport Medium 5 Gal. 625.00 Add to Cart

15560arrow12Mirsky's Fixative™

  • Saponin/Glyoxylate Fixative
  • Preserves Immunohistological activity
  • Contains no formaldehyde
  • Fast-acting

Mirsky's Fixative™ is a superior fixing agent for use in immunohistological and immunological protocols. Mirsky's contains no formaldehyde or glutaraldehyde. Therefore, it has considerably reduced toxicity and virtually no odor. Mirsky's Fixative is neutral, buffered, and isotonic (308 mOsmol). Additionally, Mirsky's Fixative does not contain toxic or hazardous buffers such as cacodylate or barbital. Hardening or shrinkage of tissue is considerably reduced.

Double reactive sites afford excellent cross-linking properties while maintaining sample enzyme activity. Samples processed in Mirsky's Fixative for light microscopy can subsequently be used in electron microscopy procedures. Special buffer systems may be used in place of the buffer provided.
Use as replacement for formalin and/or glutaraldehyde fixatives in immunohistological and immnocytological staining protocols. Tissue size is unlimited, as long as the sample is no thicker than 0.5 cm in at least one plane to assure uniformity of tissue penetration. The gross visual appearance will be different than in formaldehyde and tissue may seem "raw". This is due to double site binding of the fixative with a resultant reduction in tissue shrinkage. Microscopic examination of tissue morphology will be noticeably improved. This material is intended to maintain enzyme and antibody activity. For best results, tissue section of high digestive enzyme content should be thoroughly rinsed in saline solution before fixation (e.g. trypsin in intestinal samples). Once fixed, tissue may be retained in Mirsky's Fixative and ethanol indefinitely.

Mirsky's Fixative is normally distributed as a concentrated two bottle system, although high through-put laboratories often use the single bottle ready-to-use format. The two bottle system is comprised of Mirsky's Fixative 10X Concentrated and Mirsky's Fixative 10X Buffer. To reconstitute to working strength, add 1 part Mirsky's Fixative 10X Buffer to 8 parts distilled or deionized water, mix thoroughly, then add 1 part Mirsky's Fixative 10X Concentrate and mix again.

The two bottle system has a shelf life of 12 months while the ready-to-use format has a shelf life of 30 days.

RT 15560 Mirsky's Fixative System msds
msds
200 ml 42.00 Add to Cart
RT 15561-05 Mirsky's Fixative Ready-to-Use in Ethanol msds 500 ml 24.00 Add to Cart
RT 15561-10 Mirsky's Fixative Ready-to-Use in Methanol 1L 42.00 Add to Cart
RT 15561-20 Mirsky's Fixative Ready-to-Use in Solvent of your choice 2L 82.00 Add to Cart

msdsarrow12Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In its place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

RT 64123-05 Russell's Zenker Fixative 500 ml 27.00 Add to Cart
RT 64123-06 Russell's Zenker Fixative 1 L 46.00 Add to Cart
RT 64123-06-1G Russell's Zenker Fixative 1 Gal. 160.00 Add to Cart
RT 64123-06-5G Russell's Zenker Fixative 5 Gal. 495.00 Add to Cart

Technical Tips

The Use of Glutaraldehyde, Acrolein and Paraformaldehyde Buffer

Certificates of Analysis


Glycerine to HPMA arrow13arrow13